中文摘要：

目的：探讨H2S对巨噬细胞COX-2表达及泡沫化的影响。方法：建立氧化低密度脂蛋白（Ox-LDL）诱导RAW264.7细胞泡沫化模型,同时给予不同浓度的H2S。应用油红O染色观察泡沫细胞的形成;Real-time qPCR检测COX-2 mRNA的表达;Western blot检测COX-2蛋白的表达。结果：Ox-LDL能够明显增加细胞内脂质的沉积,同时伴有COX-2的产生。不同浓度的H2S可以明显减少由Ox-LDL引起的COX-2的表达,同时也减少了细胞内脂质的沉积,以250μmol/L最为明显,差异具有统计学意义（P〈0.05）。结论：H2S可以显著减少巨噬细胞COX-2的表达及泡沫化。

英文摘要：

Objective：To explore effects of H2S on expression of COX-2 on macrophages（RAW264.7） and formation of macrophage-derived foam cells. Methods：RAW264.7 foam cell model induced by low-density lipoprotein（Ox-LDL） is established,then added with different concentrations of H2S. Detection of foam cell formation is conventionally practiced by Oil Red O staining.The expressions of COX-2 mRNA and protein are detected by real-time qPCR and Western blot respectively. Results：A significant increase of the production of COX-2 and the deposition of intracellular lipid was observed. Different concentrations of H2S can considerably reduce the expression of COX-2 and the deposition of intracellular lipid induced by the Ox-LDL,especially the concentration of 250 μmol / L and the difference is statistically significant（P 0.05）. Conclution：H2S can significantly reduce the expression of COX-2 in macrophages（RAW264.7）and inhibit the formation of foam cells.