中文摘要：

目的探讨可诱导共刺激分子（Inducible costimulator,ICOS）信号对感染日本血吸虫小鼠的CD154/CD40表达及免疫病理的影响。方法建立ICOS转基因（ICOS transgenic,ICOS-Tg）小鼠及野生型FVB/NJ小鼠日本血吸虫病模型,应用流式细胞术、免疫组化技术分别检测感染前后的小鼠脾淋巴细胞与肝脏虫卵肉芽肿周围炎性浸润细胞CD154、CD40表达水平。应用HE染色法观察小鼠肝脏虫卵肉芽肿病变动态变化。结果与野生型FVB/NJ小鼠相比,ICOS-Tg小鼠脾淋巴细胞CD154、CD40表达水平升高,在感染后12、16周差异有统计学意义（P均〈0.05）;ICOS-Tg小鼠肝脏虫卵肉芽肿炎性浸润细胞CD154、CD40表达亦显著升高,在感染后7、12、16、20周差异有统计学意义（P均〈0.05）。且ICOS-Tg小鼠肝脏虫卵肉芽肿体积显著大于野生型小鼠（P〈0.01或0.05）。结论在日本血吸虫感染中,ICOS信号对CD154/CD40表达具有一定的调控作用,在免疫病理中起重要调控作用。

英文摘要：

Objective To explore the effect of ICOS signaling on the CD154/CD40 expressions and immunopathology in mice infected with Schistosoma japonicum. Methods ICOS transgenic（ICOS-Tg）mice and wildtype FVB/NJ mice were used as experimental schistosomiasis models. The expressions of CD154 and CD40 on splenocytes and on inflammatory cells around granulomatous infiltration of the liver in the mice infected with S. japonicum were detected by flow cytometry and immunohistochemical staining. HE staining was applied to observe the changes on the granulomatous of the mice liver. Re Results Compared with the wildtype FVB/NJ mice,the expressions of CD154 on CD4＋T splenocytes and of CD40 on CD19＋B splenocytes in the ICOS-Tg mice significantly increased in 12 and 16 weeks post-infection（all P〈0.05）. Moreover,the expressions of CD40 and CD154 on inflammatory cells around granulomatous infiltration in the liver of the ICOS-Tg mice were significantly higher than those of the wildtype FVB/NJ mice in 7,12,16 and 20 weeks post-infection（all P〈0.05）.The volumes of liver egg granulomas of the ICOS-Tg mice were significantly bigger than those of the wildtype mice（P〈0.05 or P〈0.01）. Conclusions In ICOS-Tg mice infected with S. japonicum,the ICOS signaling has a regulatory effect on CD154/CD40 expressions,and may play an important role in the hepatic egg granuloma formation of schistosomiasis.