中文摘要：

[目的]验证pinA和pinB对小麦籽粒质地的影响。[方法]将含有控制硬度的主效基因pinA和pinB的单子叶植物高效表达载体pUBPa和pUBPb，分别通过花粉管通道法将其转入小麦品种中优9507（pinB-Dlb）中。[结果]获得了转基因植株，PCR检测和基因组Southern杂交证实了外源基因在受体小麦基因组中的整合，水洗淀粉表面蛋白的定量分析表明外源基因的导入影响了friabilin表达。与非转基因对照相比，小麦籽粒质地发生变化。[结论]结果表明，PINA和PINB共同作用形成frlabilin，从而直接影响小麦籽粒质地的形成。

英文摘要：

[Objective] Grain hardness is an important factor in determining wheat end-use quality and one of the most important targets in wheat quality improvement, [Method ] In this study, two monocotyledonous expression vectors pUBPa and pUBPb harboring pinA and pinB genes, the major genes controlling grain hardness, were transferred into wheat variety Zhongyou 9507 （pinB-Dlb） via pollen-tube pathway, respectively, and transgenic plants were obtained. [ Result ] The integration of the foreign puroindolines genes was confirmed by both PCR and genomic Southern blot analysis. Quantitative analysis of the friabilin on the surface of water-washed starch granules showed that the integration of foreign puroindolines genes indeed affected the expression level of puroindolines and grain texture. [Conclusion] In conclusion, PINA and PINB （Puroindoline） interact to form friabilin and affect wheat grain texture.