中文摘要：

目的：探索不同体外刺激对小鼠腹膜腔B细胞产生IL-10的影响。方法：在不同刺激剂作用下,体外培养小鼠腹腔细胞5或48小时,流式细胞仪分析CD19＋IL-10＋B细胞比例。结果：PIM刺激5小时后,小鼠腹腔CD19＋IL-10＋B细胞比例明显升高至14.69%（P〈0.01）,在PIM刺激的基础上加入anti-IgM F（ab＇）2或anti-CD40均不能进一步提高腹腔B细胞IL-10的表达（P〉0.05）,而加入IPS后,CD19＋IL-10＋B细胞比例升高至平均26.53%（P〈0.01）。LPS、anti-CD40刺激48小时可明显升高CD19＋IL-10＋B细胞比例,但能够产生更强BCR信号的coated anti-IgM刺激抑制小鼠腹腔B细胞IL-10的表达。结论：体外刺激5小时,LPS＋PIM是腹腔产生IL-10的B细胞活化的最佳刺激,刺激48小时,LPS和anti-CD40可进一步促进腹腔B细胞IL-10的表达,这一作用可能是通过诱导腹腔B10前体细胞成熟实现的。而anti-IgM刺激对腹腔B细胞产生IL-10的影响依赖于刺激时间和信号强度。

英文摘要：

Objective ：To explore the effect of the different stimulation in vitro on IL-10 producing B ceils in peritoneal cavity of mice. Methods： Peritoneal cavity ceils of mice were cultured in vitro with different stimulator for 5 h or 48 h, then the percentage of the CD19 ＋ IL-10 ＋ B ceils was analyzed by flow cytometry. Results： The percentage of CD19＋ IL-10 ＋ B cells was raised to 14.69% with PIM stimulation for 5 h （ P 〈 0.01 ）, and the stimulation of anti-IgM F （ ab＇ ） 2 or anti-CD40 couldn＇ t promote further the expression of IL-IO of B cells in peritoneal cavity of mice（ P 〉 0.05 ）. However, the mean percentage of CD19 ＋IL-10 ＋ B ceils after LPS ＋ PIM stimu- lation for 5 h was 26.53% and higher significantly than stimulation with PIM only（ P 〈 0.01 ）. The stimulation with LPS and anti-CD40 for 48 h could further raise the expression of IL-10 of B cells in peritoneal cavity. But the coated anti-IgM stimulation Which could induce stronger BCR signal inhibited the producing of IL-10 of peritoneal cavity B cells. Conclusion ：LPS ＋ PIM is the best stimulator for the activation of IL-10-producing B cells in peritoneal cavity of mice during 5 h stimulation. LPS or anti-CD40 stimulation for 48 h can further raise the frequencies of CD19＋ IL-10＋ B ceils in peritoneal cavity of mice through inducing the mature of peritoneal cavity B10pro. However, the effect of anti-IgM stimulation on the IL-10-producing B cells in peritoneal cavity of mice is dependent on the stimulation times and signal strength.