中文摘要：

目的：探索海带多糖体内外抗血栓作用的量效关系。方法：在体外实验中，将小鼠随机分为生理盐水组、肝素4个等级剂量组、海带多糖4个等级剂量组。体外旋转法观测血栓重量，并进行各组比较。在体内实验中，将大鼠随机分为生理盐水组、阿司匹林高低剂量组、海带多糖高低剂量组、肾上腺素模型组。注射肾上腺素制备内皮损伤大鼠模型，通过动-静脉环路法测定血栓湿重。结果：体外血栓形成实验表明，与生理盐水组比较，肝素和海带多糖各剂量组均显示出抗血栓形成作用（P〈0.01），且作用随剂量的增大而增强。体内血栓形成实验表明。与模型组比较，海带多糖高剂量组、阿司匹林高低剂量组均能降低大鼠血栓湿重（P〈0.01）。显现出剂量依赖性。结论：海带多糖具有剂量依赖性的抗血栓形成作用。

英文摘要：

Objective： To explore the dose-effect of Polysaccharide extracted from Laminaria japonica Aresch （PL）. Methods： For experiment in vitro, the mice were randomly divided into saline group, four dose-grade heparin groups, four-dose-grade PL groups. A circumrotating method in vitro was used to measure the weight of thrombus and compared with each other. For experiment in vivo, rats were divided into saline group, high-dose and low0-dose aspirin groups, high-dose and low-dose PL groups and adrenaline model group. A rat model of endothelial injury was established via injecting adrenaline. An artery-vein loop was used to measure the weight of thrombus. Result： Compared with the saline group, all dose-grade groups of heparin and PL showed antithrombotic effect in vitro（P〈0.01）, which potentiate, the following dose in- crease. Compared with the adrenaline model group, high-dose PL group, high-dose and low-close aspirin groups decreased the weight of thrombus in vivo（P〈0.01）, with a dose-dependent relation. Conclusion： PL exhibited an antithrombotic effect in dose-dependent.