中文摘要：

目的：研究水溶性黑灵芝多糖（awater-solublepolysaccharidesfromGanodermaatrum，PSG）-1体内抗炎活性及对甘露糖受体（mannosereceptor，MR）表达的影响。方法：腹腔注射脂多糖（lipopolysaccharide，LPS）建立小鼠体内炎症模型，随机分为5组：正常对照组、LPS组、PSG-1（高、中、低剂量，分别为100、50、25mg/（kg·d））＋LPS组。流式细胞仪检测巨噬细胞中MR表达、吞噬功能及活性氧（reactiveoxygenspecies，ROS）生成，用酶联免疫吸附测定（enzyme-linkedimmunosorbentassay，ELISA）分析血清中肿瘤坏死因子（tumornecrosisfactor，TNF）-α、白细胞介素（interleukin，IL）-1β和IL-6的含量。结果：与正常对照组相比，LPS组腹腔巨噬细胞表面MR表达量下降，与LPS组相比，PSG-1＋LPS组腹腔巨噬细胞表面MR表达量极显著增加（P＜0.01）；与正常对照组相比，LPS组腹腔巨噬细胞吞噬能力和ROS生成增加，与LPS组相比，PSG-1＋LPS组腹腔巨噬细胞吞噬功能和ROS生成显著降低（P＜0.05，P＜0.01）；与正常对照组相比，小鼠经LPS处理后，血清中TNF-α、IL-1β和IL-6的分泌水平极显著升高（P＜0.01）；与LPS组相比，小鼠灌胃PSG-1后，PSG-1（高剂量）＋LPS组中TNF-α的含量显著降低（P＜0.05），IL-1β和IL-6的分泌水平无显著差异。结论：PSG-1具有抗炎作用，可部分抑制LPS诱导的体内炎症，其机理与PSG-1促进小鼠腹腔巨噬细胞表面MR的表达、抑制巨噬细胞向M1型极化有关。

英文摘要：

Objective:To explore the in vivo anti-inflammatory activity of a water-soluble polysaccharide purified fromGanoderma atrum,named PSG-1,and its effect on mannose receptor(MR)expression.Methods:In the present study,lipopolysaccharide(LPS)via intraperitoneal injection was applied to establish a mouse model of inflammation.Mice wererandomly divided into five groups:control group,LPS group,high-dose PSG-1(100mg/(kg·d))+LPS group,mediumdosePSG-1(50mg/(kg·d))+LPS group and low-dose PSG-1(25mg/(kg·d))+LPS group.MR expression,phagocytosisand the level of reactive oxygen species(ROS)in macrophages were analyzed by flow cytometry.The levels of tumornecrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in serum were determined by enzyme-linkedimmunosorbent assay(ELISA).Results:Compared with the control group,MR expression on the macrophage surfacewas decreased in the LPS group.In contrast,MR expression on the macrophage surface was significantly increased inthe PSG-1+LPS group in comparison with the LPS group(P<0.01).Macrophage phagocytosis and the level of ROS inthe LPS group were higher than in the control group,which were significantly decreased after administration of PSG-1(P<0.05,P<0.01).After mice were administrated with LPS,the levels of TNF-α,IL-1βand IL-6were significantlyincreased in serum in comparison with the control group(P<0.01).Compared with the LPS group,the expression levelof TNF-αwas significantly decreased after administration of high-dose PSG-1(P<0.05),but the expression levels ofIL-1βand IL-6did not significantly change.Conclusion:PSG-1had an anti-inflammatory effect,being able to partlysuppress inflammation induced by LPS in vivo,by promoting MR expression on the peritoneal macrophage surface in miceand suppressing macrophage polarization to M1phenotype.