研究了培养基MRS组分对乳酸菌Lactobacillus J23产生广谱抗菌肽Bac-J23的影响,并对其组成进行了优化。结果表明,当培养基中浓度为5g/L的乙酸钠对Bac-J23的合成具有刺激诱导作用,蔗糖对Bac-J23合成的最有利,酵母提取物对Lactobacillus J23的生长和Bac-J23的合成均具有明显的促进作用,KH2PO(415g/L~20g/L)有利于Bac-J23的合成,MgSO(40.2g/L)对合成Bac-J23最有利,MnSO4和Tween-80对Bac-J23合成及Lactobacillus J23生长均没有明显影响。
The effect of MRS medium ingredient on the bacteriocin Bac-J23 produced by Lactobacillus J23 were studied.The results showed that 5g/L acetic acid sodium,sucrose and Yeast extraction could stimulate the growth of strain Lactobacillus J23 and induce Bac-J23 production.Higher concentration KH2PO4(15g/L~20g/L)and 0.2g/L MgSO4 were propitious to Bac-J23 production,while MnSO4 and Tween-80 had no influence to the growth of Lactobacillus J23 and the production of Bac-J23.