中文摘要：

背景中国在有 000 个案例每年报导了的 30 000-50 的世界上是有肾的症候群(HFRS ) 的出血性的发烧的最严重的地方性的区域，它占世界范围的案例的超过 90% 全部的数字。在山东省的症候群的发生率是之一在中国最高，它曾经每年每 100 000 个人到达了 50。然而， hantaviruses (HV ) 的分子的特征在山东的流行病省遗体不清楚。因此澄清核苷酸顺序和在山东省孤立以便提供更好的忠告控制并且阻止 HFRS.Methods RNA 的 HV 的种系发生的特征是有用的从临床上诊断的病人和被间接 immunofluorescent 试金(IFA ) 检测的积极啮齿类动物肺的 sera 被提取。HV 的部分 M 片断与反向的抄写从 RNA 被放大用 hantavirus 遗传型的嵌套的聚合酶链反应(嵌套的 PCR ) 特定的教材。嵌套的 PCR 产品被定序并且与那些相比从以前，流行病从 GenBank 在山东并且与另外的代表性的 HV 序列孤立。种系发生的树分析基于 34 HV 在山东孤立的 M genes.Results 的序列被执行显示出的 67.1%100% 核苷酸身份。在 6 个 Hantaan 病毒(HTNV ) 之中的核苷酸相同在山东孤立是 78.1%-98.7% ，当病毒(SEOV ) 在山东孤立的在 28 汉城之中的相同是 93.7%100% 时。有至少 3 子类型 HTNV ( H2 ， H5 ， H9 )并且 2 子类型 SEOV ( S2 ， S3 )在在山东省的山东 Province.Conclusions ， HTNV 的相同更低，没有占优势的子类型，当 SEOV 的相同更高， S3 是占优势的子类型时。从啮齿类动物的 SEOV 的相同比从病人的那些高。在山东的子类型的分发类似于毗连的省的。序列的种系发生的分析显示出在山东的 HV 的地理聚类。

英文摘要：

Background China is the most severe endemic area of hemorrhagic fever with renal syndrome （HFRS） in the world with 30 000-50 000 cases reported annually, which accounts for more than 90% of total number of cases worldwide. The incidence rate of the syndrome in Shandong Province is one of the highest in China, which has ever reached 50 per 100 000 persons per year. However, the molecular characteristics of hantaviruses （HV） epidemic in Shandong Province remain unclear. Therefore it is useful to clarify nucleotide sequence and phylogenetic characteristics of HV isolated in Shandong Province in order to provide better advices to control and prevent HFRS. Methods RNAs were extracted from sera of clinically diagnosed patients and positive rodent lungs that were detected by indirect immunofluorescent assay （IFA）. Partial M segments of HV were amplified from the RNAs with reverse transcription nested polymerase chain reactions （nested PCR） using hantavirus genotype specific primers. The nested PCR products were sequenced and compared with those from previously epidemic isolates in Shandong and with other representative HV sequences from GenBank. Phylogenetic tree analyses were performed based on the sequences of the M genes. Results Thirty-four HV isolates in Shandong showed 67.1%-100% nucleotide identities. The nucleotide homologies among 6 Hantaan viruses （HTNV） isolates in Shandong were 78.1%-98.7%, while the homologies among 28 Seoul virus （SEOV） isolates in Shandong were 93.7%-100%. There were at least 3 subtypes HTNV （H2, H5, H9） and 2 subtypes SEOV （S2, S3） in Shandong Province. Conclusions In Shandong Province, the homologies of HTNV were lower and there were no predominant subtypes, while the homologies of SEOV were higher and S3 was the predominant subtype. The homologies of SEOV from rodents were higher than those from patients. The distribution of subtypes in Shandong was similar to that of the adjoining provinces. Phylogenetic analyses of the sequences showed geographic