中文摘要：

目的制备马钱子碱白蛋白纳米粒,并考察其体外理化性质。方法采用去溶剂化-化学交联法制备白蛋白纳米粒。利用透射电镜、马尔文激光粒度仪、HPLC法及考马斯亮兰-酶标仪对制备的纳米粒进行表征。结果制得的纳米粒呈类球形,平均粒径为（209.8±3.6）nm,Zeta电位（-34.49±1.32）mV,纳米粒收率90.0%±3.2%,包封率为60%±2.3%,载药量为2%±1.2%,体外模拟释药结果表明载药纳米粒药物释放速率在24h内持续稳定。结论去溶剂化-化学交联法制备马钱子碱白蛋白纳米粒简便可靠,体外释药具有明显的缓释作用。

英文摘要：

OBJECTIVE To prepare Brucine-BSA-NPs and to observe its physicochemical property in vitro.METHODS Brucine-BSA-NPs was prepared by using desolvation-crosslinking method,and characterized by TEM,Zetasizer 3000HS,HPLC,and Coomassie brilliant blue-enzyme-labeled instrument.RESULTS The prepared Brucine-BSA-NPs was round with average diameter of（209.8±3.6） nm,Zeta potential of（-34.49±1.32） mV,90.0%±3.2% yield,encapsulation rate of 60%±2.3%,and drug loading of 2%±1.2%.The results of modeling drug release in vitro illustrated the releasing rate of Brucine-BSA-NPs with loaded drug was continuously stable in 24 h.CONCLUSION Applying desolvation-crosslinking method to prepare Brucine-BSA-NPs is convenient and reliable,showing conspicuous sustained release of drug in vitro.