中文摘要：

目的比较2型糖尿病大鼠和正常大鼠在急性败血症合并应激性高血糖后的炎症反应程度和氧化应激的损伤情况。方法将26周龄2型糖尿病GK大鼠和普通Wistar大鼠腹腔注射5mg／kg脂多糖构建大鼠急性败血症模型。比较脂多糖注射前后2种大鼠血清中促炎因子肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1β、IL-6和抗炎因子IL-10水平；化学比色法测定各组大鼠血清和脏器组织（肺脏、肝脏、肾脏、心脏）丙二醛、总抗氧化能力（T—AOC）水平。结果在基线水平，GK大鼠的促炎因子水平（TNF-α、IL-6、IL-10）高于普通大鼠，注射脂多糖后，GK大鼠促炎因子（TNF—α、IL-6）上升幅度明显小于Wistar大鼠（P〈0．05），而抗炎因子（IL-10）上升幅度则明显高于Wistar大鼠；在基线水平，GK大鼠血清的丙二醛水平显著高于Wistar大鼠[（25．76对12．71）μmol／L，P〈0．05]，而血清T—AOC水平则明显低于Wistar大鼠[（0．60对2．8）U／ml，P〈0．05]，脂多糖注射1h后2种大鼠血清丙二醛含量较注射前显著升高[（32．30和20．19）μmoL／L]，T—AOC水平[（0．20和2．08）U／m1]均较基线水平明显下降（P〈0．05），且2种大鼠的变化幅度没有明显差异（P〉0．05），2种大鼠各脏器组织（肺脏、肝脏、肾脏、心脏）的丙二醛、T—AOC水平在脂多糖注射前后差异均无统计学意义（P〉0．05）。结论2型糖尿病大鼠与普通大鼠相比，在急性败血症状态下促炎因子释放减少，抗炎因子释放增加，对急性炎症反应耐受性更高，引起炎症失控性扩散的可能性更小．

英文摘要：

Objective To compare the differences in responsive ability and oxidative stress damage between type 2 diabetic rats and normal rats under acute sepsis associated with stress hyperglycemia. Methods GK rats with type 2 diabetes and Wistar rats were established critical ill models of sepsis by intraperitoneal injection of 5 mg/kg lipopolysaccharide（LPS）. Before and after LPS injection, serum levels of proinflammatory cytokines tumor necrosis factor α （TNF-α）, interleukin （IL）-1β, and IL-6, and anti-inflammatory cytokine IL-10 were determined. Malondialdehyde （MDA） and total antioxidative capacity （ T-AOC ） levels in serum and tissues （ lung, liver, kidney, heart） were measured by colorimetric determination. Results Before LPS injection, serum levels of TNF-α, IL-6, and IL-10 in GK rats were higher than those of Wistar rats. The serum levels of TNF-α, IL-6, and IL-10 after LPS injection were raised in both GK rats and Wistar rats （ all P〈0.05 ）. At baseline, serum MDA level of GK rats was higher than that of Wistar group [ （ 25.76 vs 12.71 ） μmol/L, P〈0.05 ], while T-AOC level were lower [ （ 0.60 vs 2.8 ） U/ml, P〈0.05 ]. One hour after LPS injection, the serum MDA level in two kinds of rats significantly increased compared with those of their baseline levels[ （ 32.30 and 20.19 ） μmol/L, both P〈0.051 , while their serum T-AOC level decreased [ （0.20 and 2.08 ） U/ml, P〈0.051 ）- There were no signigicant differences in the change trend of serum MDA and T-AOC between the two kinds of rats. MDA and T-AOC levels in tissues had no significant difference before and after LPS injection in GK and Wistar rats （ P 〉 0.05 ）. Conclusions The rats with type 2 diabetes had lowered level of proinflammatory factors and raised level of anti-inflammatory factors under acute sepsis, presenting better tolerance and lowered probability of inflammatory diffusion compared with normal rats. ]