中文摘要：

【目的】明确桔小实蝇Bactrocera dorsalis（Hendel）飞行肌对能源物质的利用。【方法】通过生化方法测定了能源物质代谢相关5种酶[3-磷酸甘油醛脱氢酶（GAPDH）、3-磷酸甘油脱氢酶（GDH）、乳酸脱氢酶（LDH）、柠檬酸合酶（CS）和3-羟酰辅酶A脱氢酶（HOAD）]活性的变化。【结果】桔小实蝇成虫中所测的5种酶活性随日龄的变化而变化,4日龄GAPDH,GDH,LDH和CS活性最高,20日龄HOAD活性最高。吊飞过程中,GAPDH,GDH和CS的活性变化基本一致,随吊飞时间的延长活性逐渐升高;LDH和HOAD的活性变化雌、雄虫完全不同。雄虫LDH活性除吊飞2 h外其他时间均高于静息状态,雌虫则始终低于静息状态;雄虫HOAD活性只有吊飞24 h低于静息状态水平,而雌虫吊飞后HOAD活性一直在静息状态水平及以下波动。【结论】桔小实蝇飞行所利用的能源物质包括糖类和脂肪,以糖类能源为主。吊飞过程中,雄虫除可以进行高速有氧代谢以外,还具备一定的无氧代谢能力,而雌虫只进行有氧代谢;雄虫能利用脂肪供给能量,雌虫则几乎不动用脂肪。研究结果为进一步阐明桔小实蝇的迁飞行为机制提供了依据。

英文摘要：

【Aim 】This study aims to clarify the utilization of energy substances in flight muscles of Bactrocera dorsalis（ Hendel）. 【Methods】The activities of five key enzymes related to energy metabolism in flight muscles of B. dorsalis,i. e.,glyceraldehyde phosphate dehydrogenase（ GAPDH）,glycerol-3-phosphate dehydrogenase（ GDH）, lactate dehydrogenase（ LDH）, citrate synthase（ CS） and 3-hydroxyacyl-Co A dehydrogenase（ HOAD）,were assayed and analyzed by tethered flight testing and biochemical methods in the laboratory. 【Results】The activities of the five enzymes assayed changed with age（ day-old） of B. dorsalis adults. The activities of GAPDH,GDH,LDH and CS peaked in the 4-dayold adults,while the HOAD activity peaked in the 20-day-old adults. During tethered flight,the activities of GAPDH,GDH and CS showed similar change trend,gradually increasing with tethered flight duration,while the activities of LDH and HOAD showed distinctively different change trend between male and female adults. The LDH activity in female adults during tethered flight was lower than that of female adults in resting state,while that in male adults was just the opposite except the 2 h tethered flight treatment. The HOAD activity in male adults fluctuated above the resting state level except the 24 h tethered flight treatment,while that in female adults fluctuated between the resting state level and below.【Conclusion】Energy substrates in flight muscles of B. dorsalis include carbohydrate and lipid,butcarbohydrate metabolism provides primary energy. During tethered flight,significantly different energy metabolisms exist between male and female adults. Both aerobic and anaerobic metabolisms are present in flight muscles of male adults,but only aerobic metabolism is present in female adults. Male adults can utilize lipids as energy substrate for flight,but female adults hardly utilize lipids as energy substrate for flight. Our study provided a scientific basis for further illuminating the mechanisms of the migra