中文摘要：

目的 观察小鼠骨髓间充质干细胞（MSC）在肝脏特定微环境下是否向具肝细胞表型与功能的细胞横向分化，并探讨其治疗肝功能损伤的可行性。方法 20只裸小鼠，随机分为4组，每组5只：A组：CCl4与橄榄油1：1混合，按1ml／kg腹腔注射，每周2次。1周后经尾静脉移植1×10^6GFP阳性MSC，术后继续注射CCl4，每周2次；B组：CCl4注射同A组，经尾静脉注入等量生理盐水；C组：正常小鼠，细胞移植同A组；D组：正常对照。术后4周处死全部受试动物，血清检测肝功能，HE、Masson染色行组织学观察，双荧光染色确认GFP阳性细胞并观察其是否表达肝细胞特异性蛋白。结果 A、B组受体肝纤维组织增生显示肝功能损伤模型制作成功，二组胶原分布百分比差异并无统计学意义（10．5±1．5vs12．7±1．6，t=-2．238，P〉0．05）。A组镜下可见GFP阳性细胞主要分布于汇管区及小叶间隔周围，部分同时表达白蛋白（35％±7％）；B、C、D组镜下均未见绿色荧光细胞。A组白蛋白水平明显高于B组（24．4g／L±3．3g／Lvs18．6g／L±2．9g／L，P〈0．05），两组血清丙氨酸转氨酶（ALT）水平差异有统计学意义（121U／L±21U／Lvs192U／L±29U／L，P〈0．05）。结论 持续肝功能损伤可以有效诱导MSC向肝迁移增殖，部分MSC在肝细胞持续损伤的微环境下有向肝样细胞横向分化的潜能，提示MSC移植可以在一定程度上修复受损肝功能。

英文摘要：

Objective To observe whether bone mesenehymal stem cells （MSCs） have the potential to transdifferenfiate into functional hepatocyte-like cells in the special ＂niche＂ as well as the therapeutic feasibility to repair damaged liver in mice. Methods 20 nude mice were randomly divided into four groups （n =5 in each group） ： Group A： 1.0 ml/kg of carbon tetrachloride（CCl4） （dessolved in olive oil by ratio of 1：1 ） was injected into the peritoneum of mice twice a week for 5 weeks. GFP-positive MSCs（ 1 ×10^6 cells） were injected into the caudal tail vein 1 week after the first dose of CCl4 ;Group B： treated with CCl4 as in A, but received the same volume of saline ; Group C ： normal nude mice with GFP-positive MSCs Transplanted in the same way as in A. Group D ： normal controls. 4 weeks after the cell transplantation, all animal subjects were killed. Liver function tests （LFT） , histology of HE and Masson staining as well as double immunofluorescent staining for GFP and albumin were studied in all groups. Results The hepatic fibrosis in group A ＆ B confirmed the success of model for liver damage and there was no marked difference in the percent of the area occupied by collagen between two groups（ 10.5 ± 1.5 vs 12. 7 ± 1.6,t = -2. 238 ,P 〉 0. 05）. GFP-positive MSCs were mainly observed around portal area or interspace of lobules in group A. Some of GFP-positive cells also express albumin（35% ＋7% ）. While in group B,C or D,there is no such findings. The level of serum albumin in group A was higher than that in group B （ 24. 4 g/L ±3.3 g/L vs 18.6 g/L ±2. 9 g/L,P 〈0. 05） while the level of ALT was also different between two groups （ 121 U/L±21 U/L vs 192 U/L ± 29 U/L, P 〈 0.05 ） . Conclusion The stimulus of persistent liver damage might enhances the migration of MSCs to the liver , in which some of the MSCs have the potential to transdifferentiate into hepatocyte-like cells. Transplantation of MSCs might amend the damaged tissue of host liver to a cert