中文摘要：

没有 DNA 抽取， conchocelis 的小片断直接在被放大的 PCR 的一个改进方法被用来放大 RUBISCO 内部从红海藻的类 Porphyra 的九种的遗传因子的分隔符 DNA 碎片(Bangiales， Rhodophyta ) ，包括 Porphyra yezoensis (江苏，中国) ， P。haitanensis (福建，中国) ， P。oligospermatangia (Qingdao，中国) ， P。katadai (Qingdao，中国) ， P。tener 一(Qingdao，中国) ， P。suborboculata (福建，中国) ， P。pseudolinearis (Kogendo，朝鲜) ， P。线性(Devon，英格兰) ，并且 P。秋天斧子(美国西雅图) 。这里开发的标准 PCR 和方法两个都用为 RUBISCO 分隔符区域，二个 PCR 产品在以后被定序特定的教材被进行。用两个方法获得的 amplicons 的定序的数据是相同的，建议改进 PCR 方法是功能的。这些调查结果显示这里开发的方法可能为在一个细菌原生质银行的 Porphyra 的种类的快速的鉴定是有用的。另外，一棵种系发生的树用 RUBISCO 分隔符和部分 rbcS 顺序被构造，并且结果在与可能的其他的发展史一致基于传统的词法分类特征，显示 RUBISCO 分隔符是为种系发生的研究的一个有用区域。

英文摘要：

An improved method of PCR in which the small segment of conchocelis is amplified directly without DNA extraction was used to amplify a RUBISCO intergenic spacer DNA fragment from nine species of red algal genus Porphyra （Bangiales, Rhodophyta）, including Porphyra yezoensis （Jiangsu, China）, P. haitanensis （Fujian, China）, P. oligospermatangia （Qingdao, China）, P. katadai （Qingdao, China）, P. tenera （Qingdao, China）, P. suborboculata （Fujian, China）, P. pseudolinearis （Kogendo, Korea）, P. linearis （Devon, England）, and P. fallax （Seattle, USA）. Standard PCR and the method developed here were both conducted using primers specific for the RUBISCO spacer region, after which the two PCR products were sequenced. The sequencing data of the amplicons obtained using both methods were identical, suggesting that the improved PCR method was functional. These findings indicate that the method developed here may be useful for the rapid identification of species of Porphyra in a germplasm bank. In addition, a phylogenetic tree was constructed using the RUBISCO spacer and partial rbcS sequence, and the results were in concordant with possible alternative phylogenies based on traditional morphological taxonomic characteristics, indicating that the RUBISCO spacer is a useful region for phylogenetic studies.