中文摘要：

目的探索影响朝鲜淫羊藿种子萌发影响因子。方法采用显微测量、TTC染色法和石蜡切片研究了朝鲜淫羊藿种子在贮藏过程中胚率、胚活力和结构的变化；通过生物鉴定法检测发芽抑制物；离体胚培养法诱导愈伤组织。结果朝鲜淫羊藿种子属于胚形态后熟，需经过一定时间的层积促使胚成熟；种子中含有一定活性的萌发抑制物质，但其对白菜幼根生长的抑制活性高于对白菜种子萌发的抑制活性；成熟离体胚在1／2MS＋NAA0．4mg·L-1。＋6-BA1．0mg·L-1培养基上诱导较好。结论朝鲜淫羊藿种子透水性良好，影响种子萌发的主要原因是胚形态后熟，变温层积处理可加速胚的发育，胚乳中存在萌发抑制物。

英文摘要：

Objective To investigate factors influencing seed germimation of Epimedium koreanum Naked. Methods Micrometering, TTC staining and paraffin section were used to observe the variation of embryo rate, embryo viability rate and structure. And the biological test was applied for detecting the germination in- hibitor. In vitro culture of maturated embryo was employed to seedlings and callus. Results E. koreanum Na- ked seeds belongs to embryo afterripening, it needs some stratification time to make it mature. The activity of intrinsic inhibitor exists in the seeds, and both of the intrinsic inhibitors showed stronger inhibitory effect on the growth of younger roots of Chinese cabbage than that on their seed germination. The optimum culture medium for maturated embryo was 1/2 MS ＋6-BA 1.0 mg-L-1 ＋ NAA 0.4 mg.L-1. Conclusions After ripening of embryogenesis contribute mainly to the dormancy of the E. koreanum Naked seeds. By changing the temperature stratification, most embyo of seeds are maturated followed by the existence of germination inhibitor in the endosperm.