[目的]研究草莓炭疽病(Colletotrichum fragariae)的生物防治方法。[方法]采用菌丝生长抑制法测定枯草芽孢杆菌( Bacillus subtilis)(BS)和蛇床子素(SC)对草莓炭疽病菌的室内抑菌活性,并进行田间防治试验。[结果]室内测定结果表明,BS和 SC对草莓炭疽病菌菌丝生长都有极强的抑菌活性,其 EC50值分别为0.0075和1.0630 ml/L。田间防治结果显示,无论在避雨或露天条件下,BS(用量600-750 g/hm2)和25%咪鲜胺 EC (用量600 ml/hm2),在防治3次后7和14天的防治效果均达76%以上,但两药剂与SC(用量1800-2700 ml/hm2)的田间防治效果间有极显著差异(P〈0.01)。[结论]枯草芽孢杆菌可以用于草莓炭疽病的防控。
[Objective] The aim was to explore biocontrol approaches of strawberry an-thracnose. [Method] With hyphal growth inhibition method, bacteriostatic activities of Bacil us subtilis and Osthole on strawberry anthracnose were measured and field test was carried out. [Result] The results show that both of Bacil us subtilis and Osthole were of higher bacteriostatic activity on strawberry anthracnose, and the values of EC50 were 0.007 5 mg/L and 1.063 0 ml/L, respectively. The result of field test show that the prevention effects of Bacil us subtilis (600-750 g/hm2) and 25% prochloraz (600 ml/hm2) both achieved higher than 76%, 7 and 14 d after triple medical applica-tions with rains sheltered or in open field. But the effects were of extremely signifi-cant differences with that of Osthole (1 800-2 700 ml/hm2) (P〈0.01). [Conclusion] Bacil us subtilis can be made use of for control ing strawberry anthracnose.