中文摘要：

目的：探讨Wnt10b对Ha Ca T表皮细胞迁移的影响及机制。方法：分别用重组腺病毒Ad-GFP、Ad-GFP-Wnt10b感染Ha Ca T表皮细胞,利用细胞免疫荧光技术检测腺病毒Ad-GFP-Wnt10b感染细胞后的过表达情况;采用细胞划痕实验,于不同时间点观察并记录经Wnt10b处理后,Ha Ca T细胞体外迁移功能的改变;进一步采用Westen blot技术检测不同腺病毒处理后,Ha Ca T细胞中Wnt信号关键分子β-catenin、细胞粘附分子E-cadherin表达的改变情况。结果：1Ad-GFP-Wnt10b感染Ha Ca T细胞48 h后,细胞内GFP表达上调,细胞免疫荧光染色显示Wnt10b处理组高表达Wnt10b蛋白;2Ha Ca T细胞经Ad-GFP-Wnt10b处理后,细胞创面愈合速度明显增快;3Wnt10b处理组细胞β-catenin蛋白表达水平显著高于对照组,而E-cadherin蛋白表达水平显著低于对照组。结论：Wnt10b能促进Ha Ca T细胞迁移,且该效应涉及经典Wnt/β-catenin信号的激活及由E-cadherin介导的细胞粘附性的减弱。

英文摘要：

Objective： To research the effect and the related mechanism of Wnt10b on the migration of HaCaT epidermal cells. Methods： After the HaCaT epidermal cells were treated with different adenovirus （ Ad-GFP or Ad-GFP-Wnt10b）, the over-expression levels of Wnt10b protein in the cells were examined by immunofluorescence technique. The migration ability of HaCaT cells was detected by scratch assay at different time points. Western blot was used to measure the expression levels of β-catenin, which is the key Wnt signaling molecule, and the cell adhesion molecules E-cadherin. Results： ① The HaCaT cells were successfully infected by Ad-GFP-Wnt10b. After 48 h, the expression level of GFP in the cells increased. The Wntl0b protein was prominently located in the Wnt10b treatment cells shown by the immunofluorescence staining. ②The scrape wound of HaCaT cells healed more quickly in the Ad-GFP-Wnt10b treatment group. ③In comparison with the control group, the expression of β-catenin protein markedly increased in the Ad-GFP-Wnt10b treatment group. However, the expression of E-cadherin protein was just the opposite. Conclusions： Wnt10b may promote the migration of HaCaT cells, which involved with the activation of the canonical Wnt/β-catenin signaling and the decline of the cell adhesion ability mediated by E-cadherin.