中文摘要：

目的：细胞融合是细胞生物学中一种常用的技术,有着广泛的应用,如单克隆抗体制备,核质研究,疫苗研发等。其中,聚乙二醇（PEG）化学融合是最为常用的一种细胞融合技术,影响PEG化学细胞融合效果的因素有很多,但是对一些具体因素的研究的不是很全面。本文旨在为了更全面的了解PEG诱导的化学细胞融合的影响因素,优化融合条件,以此扩大PEG化学融合应用范围。方法：以鸡血血红细胞为材料,通过调节已有的Hanks融合液中镁离子浓度,比较各实验组以及对照组的细胞融合率,探究了Mg^2＋浓度对细胞融合效果的影响,确定了为提高细胞融合效率应使用的Mg^2＋浓度区间。结果：可以在原有的Hanks配方的基础上,调节Mg^2＋浓度至10 mmol/L-20 mmol/L这个范围,细胞融合率较大。结论：Mg^2＋对细胞融合有一定影响,通过调节Mg^2＋浓度至上述合适区间可以达到较高的细胞融合率,从而为PEG化学融合提供了一种优化方案。

英文摘要：

Objective： Cell fusion is one of the cytological techniques, which is now widely used in many fields, for instance, monoclonal antibody preparation, nucleoplasm research and vaccine development etc. Polyethylene glycol （PEG） chemical fusion is the most common form of cell fusion technology, and there are a lot of the effects of factors affect the PEG chemistry cells fusion, but some of these specific factors are not studied comprehensively. In order to more fully understand the chemistry of PEG -induced cell fusion influencing factors, we optimized fusion conditions, and expanded the range of applications of PEG chemical fusiou. Methods： Chicken red blood cells in liquid magnesium ion concentration, integration of by adjustable existing Hanks. Compared with the rate of cell fusion between each experimental group and the control group, the effect ofMg^2＋ concentration on the cell fusion was explored, and this results should be used to improve the efficiency of cell fusion. Results： Based on the original Hanks recipe, we adjusted the concentration range of Mg^2＋ to 10 mmol/L-20 mmol/L and it reached a higher rate of cell fusion. Conclusion： Mg^2＋ concentration has an effect on cell fusion, by adjusting the concentration according to the range we have just showed it can reach a higher rate of cell fusion and provide an optimization program for the PEG chemical fusion.