采用化学共沉淀法,以FeCl2·4H2O和FeC13·6H2O为原料制备磁性Fe3O4纳米颗粒(MNPs),采用氨基酸对其进行修饰使其表面连上氨基.用巯基乙酸作为稳定剂合成了水溶性的CdSe/CdS量子点,并利用偶联剂1-乙基-3-(3-二甲氨丙基)碳二亚胺盐酸盐和N-羟基琥珀酰亚胺(NHS)连接氨基修饰的Fe3O4和带有羧基的CdSe/CdS,成功制备了Fe3O4/CdSe/CdS荧光磁性纳米颗粒.该荧光磁性纳米复合物保留了CdSe/CdS量子点优异的荧光特性,平均粒径在40nm左右;磁化曲线表明此纳米复合物具有超顺磁性.这种双官能复合纳米颗粒有望成为新型荧光磁性双标记复合微粒而广泛应用于免疫检测、荧光追踪、磁性分离等领域.
In this study, the preparation of a new kind of magnetic and luminescent Fe3O4/CdSe/CdS nanocomposites was demonstrated. Superparamagnetic Fe3O4 nanoparticles were first synthesized by hydrothermal coprecipitation of ferric and ferrous ions, followed by the modification of their surfaces with L-aspartic acid. The surface-modified Fe3O4 nanoparticles were then linked to CdSe/CdS quantum dots (QDs), which were modified with mercaptoacetic acid, to form the Fe3O4/CdSe/CdS fluorescent magnetic nanocomposites through the coordination of the amino groups on the surfaces of Fe3O4 and the carboxyl groups on CdSe/CdS QDs in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). The structure and properties of as-synthesized nanocomposites were charac-terized by XRD, EDS, TEM, vibrating sample magnetometer (VSM) and photoluminescence (PL). It was indicated that the nanocomposites have a typical diameter of 40 nm and possess not only magnetic properties but also photoluminescence properties. The synthesized bifunctional nanocomposites could be used in a variety of bioanalytical assays such as luminescence detection, magnetic separation, drug delivery, biotech- nology/biomedicine and so on.