中文摘要：

目的分离鉴定黑老虎中倍半萜类化合物,并考察化合物对一氧化氮（NO）释放的抑制作用。方法采用丙酮提取,半制备高效液相色谱等方法分离化学成分,波谱方法鉴定化合物的结构;脂多糖（LPS）和γ干扰素（IFN-γ）诱导的单核巨噬细胞RAW264.7作为细胞炎症模型,考察化合物对NO释放的抑制作用。结果分离得到了4个倍半萜类化合物,经波谱方法鉴定,分别为匙叶桉油烯醇（1）、（-）-7βH-柳杉二醇（2）、（-）-依兰油醇（3）、（＋）-顺-菖蒲烯（4）。化合物1较强抑制LPS/INF-γ诱导NO的产生。结论化合物1~4均首次从南五味子属中分离得到,化合物1具有抗炎活性。

英文摘要：

Objective To study the sesquiterpenes from kadsura coccinea and their nitric oxide（NO） inhibitory activities. Methods Acetone was used to extract, the compounds were isolated by semi-preparative HPLC method, their structures were identified by spectroscopy. The activated RAW 264.7 macrophages induced by LPS and IFN-γ were used to evaluate the inhibitory activities on NO production. Results Four sesquiterpenes were isolated and identified as ent-spathulenol,（-）-7βH-eudesmane-4α, 11-diol,（-）-T-muurolol,（＋）-cis-Calamenene. Compound 1 had strong inhibitory activity on NO production. Conclusion Compound 1-4 are obtained from the Kadsura for the first time.Compound 1 has the anti-inflammatory effect.