中文摘要：

为研究鸡p15^INK4B蛋白的功能及为制备此蛋白的单克隆抗体提供材料，应用引物搭桥法合成p15^INK4B基因，应用基因工程的手段构建p15^INK4B基因的原核表达载体，在E．coli Rosetta（DE3）进行融合表达，并对表达产物进行亲和层析纯化。结果成功地构建了p15^INK4B基因原核表达载体，经亲和层析制备了较高纯度的目的表达产物。

英文摘要：

To study p15^INK4B protein and prepare its monoclonal antibody, p15^INK4B gene was obtained by overlap-ligation PCR and cloned into expression vector. Then p15^INK4B gene was expressed in E. coli Rosetta（DE3） and p15^INK4B fusion protein was purified by aflqnity chromatography.The results indicated that the recombinant expression vector pET28a（ ＋ ）-p15 was successfully constructed and relatively pure target protein was obtained.