中文摘要：

目的：探讨近红外量子点（NIR QDs）与含有精氨酸-甘氨酸-天冬氨酸序列（RGD）肽段连接的NIR QDs-RGD探针对小鼠的毒性作用,阐明NIR QDs-RGD作为一种新型肿瘤治疗手段的生物安全性。方法：用NIR-QDS与RGD肽段连接,制备探针;将24只BALB/C小鼠随机分为4组,单次注射组在第1天通过尾静脉注射100μL的QD800-RGD（含200pmol的QD800）;单次注射对照组在第1天通过尾静脉注射100μL的PBS;重复注射组分别在第1天和第7天通过尾静脉各注射100μL的QD800-RGD（含200pmol的QD800）;重复注射对照组分别在第1天和第7天通过尾静脉各注射100μL的PBS。注射14d后行毒性检测,包括小鼠全血细胞（白细胞、红细胞、血红蛋白、血小板、淋巴细胞和中性粒细胞）计数和血清生化分析（总蛋白、白蛋白、白球比、天门冬氨酸氨基转换酶、丙氨酸氨基转换酶和血尿素氮）,计算小鼠肝、脾、肾和肺的脏器系数,检测肝、脾、肾和肺组织中超氧化物歧化酶（SOD）、谷胱甘肽（GSH）活性和丙二醛（MDA）含量,并进行组织病理学检查。结果：与各自对照组比较,单次注射组小鼠脏器系数稍有降低,重复注射组小鼠脏器系数增高,但4组小鼠之间比较差异无统计学意义（P〉0.05）。全血细胞计数,与对照组比较,单次注射组小鼠白细胞、红细胞、血红蛋白、血小板、淋巴细胞数都有所增高,中性粒细胞数未见明显变化;重复注射组小鼠红细胞、血红蛋白、淋巴细胞增高,白细胞、血小板减少,中性粒细胞无明显变化;4组之间比较差异无统计学意义（P〉0.05）。血清生化指标检测,与对照组比较,单次注射组小鼠总蛋白、白蛋白、白球比、血尿素氮减少,天门冬氨酸氨基转换酶和丙氨酸氨基转换酶增加;重复注射组小鼠总蛋白、白蛋白增加,白球比、血尿素氮减少,天门冬氨酸氨基转换酶和丙氨酸氨基转换酶降低;4组之间比较?

英文摘要：

Objective To study the toxicity of near-infrared quantum dots（NIR QDs）linked to peptide containing the arginine-glycine-aspartic acid（RGD）sequence（NIR QDs-RGD）in the mice,and to clarify the biological safety of NIR QDs-RGD as a novel tumor treatment method.Methods The probe was prepared firstly by linking NIR QDs to the RGD peptide.Twenty-four BALB/C mice were randomly divided into four groups.In QD800-RGD one injection group,the mice were injected via tail vein with 100μL QD800-RGD（equivalent to 200 pmol of QD800）on the first day;in PBS one injection group,the mice were injected similarly with 100μL PBS;in QD800-RGD two injection group,the mice were repeatedly injected via tail vein with 100μL QD800-RGD（equivalent to 200 pmol of QD800）on the first and seventh days;in PBS two injection group,the mice were repeatedly injected similarly with100μL PBS on the first and seventh days.Fourteen days after injection,toxicity tests were performed,including complete blood cells（white blood cells,red blood cells,hemoglobin,platelets,lymphocytes,and neutrophils）count and serum biochemical analysis（total protein,albumin,albumin/globulin,aspartate aminotransferase,alanine aminotransferase,and blood urea nitrogen）.The coefficients of liver,spleen,kidney,and lung weight to body weight were calculated,and the activities of superoxide dismutase（SOD）,glutathione（GSH）,and malondialdehyde（MDA）contents in liver,spleen,kidney and lung tissues of the mice were detected.The histopathological examination was performed.Results Compared with PBS injcetion groups,the organ coefficients of the mice in QD800-RGD one injection group were decreased slightly,and the organ coefficients of the mice in QD800-RGD two injection group were increased,but the differences between four groups were not statistically significant（P〉0.05）.Compared with PBS injcetion groups,the number of leukocytes,erythrocytes,lymphocytes and the levels of hemoglobin and platelets were increased,and the number of neutrophils had