普通番茄和转反义L以,L2基因番茄不同成熟度果实研究结果表明。转反义LeEIL2基因番茄有效抑制了LeEIL-2基因的表达,其果实和叶片表现出与普通番茄不同的生理特性:采后果实的呼吸强度和乙烯释放量受到抑制,离体叶片的伤乙烯释放量明显降低;果实中ACC合成酶与ACC氧化酶活性以及ACC含量明显下降.从而抑制了乙烯的生物合成途径,延缓了果实的成熟衰老进程。
Fruits and leaf of anti-sense LeEIL2 tomato ((Lycopersicon esculentum Mill. cv. Zhong Shu 4th) shown differences in ethylene biosynthesis and fruit ripening. Compared with CK, respiration rate and ethylene production rate in anti-sense LeEIL2 tomato fruit were inhibited; leaf wound ethylene from anti-sense tomato decreased to 41%. Further research confirmed that ACS and ACO activities in anti-sense LeEIL2 tomato fruit decreased dramatically, which resuited in ACC content decrease, ethylene biosynthesis inhibition and eventual fruit ripening delay,