中文摘要：

目的：克隆表达H：z66阳性伤寒沙门菌鞭毛素基因fljB：z66,并纯化制备相应的多克隆抗体。方法：利用PCR技术从H：z66阳性伤寒沙门菌基因组DNA中得到鞭毛素基因fljB：z66,将该基因克隆到表达载体pET-28 a（＋）上并在大肠埃希菌JM109中进行表达;fljB：z66的表达产物经Ni柱纯化后作为抗原免疫兔以制备多克隆抗体。结果：经PCR及序列分析证实,伤寒沙门菌鞭毛素基因fljB：z66被成功导入表达载体pET-28 a（＋）,并在大肠埃希菌JM109中获得了高效表达,以此作为抗原成功制备了兔抗z66的多克隆抗体。结论：成功克隆表达了H：z66阳性伤寒沙门菌鞭毛素基因fljB：z66,并制备了相应的多克隆抗体,为今后深入研究H：z66阳性伤寒沙门菌的单向相变换机制奠定了基础。

英文摘要：

Objective： To clone and express the flagellin gene fljB：z66 of H：z66 positive Salmonella enterica serover Typhi,and prepare the anti-z66 polyclonal antibodies.Methods： Specific primers were designed to amplify the flagellin gene fljB：z66 from the chromosome DNA of H：z66 positive Salmonella enterica serover Typhi GIFU10007.The amplicon was inserted into the expression vector pET-28a（＋）,which was then transferred to E.coli JM109 to be expressed.The recombinant protein FljB-His6 was purified with Ni-TED packed column and was used as immunogen to prepare the polyclonal antibody. Results： PCR andsequencing analysis demonstrated that the flagellin gene fljB：z66 of H：z66 positive Salmonella enterica serover Typhi was inserted into the vector pET-28a（ ＋ ） and was expressed highly in E. coli JM109. The rabbit anti-z66 polyclonal antibody was prepared successfully. Conclusion： This study was a foundation to research the mechanism underlying the the unidirectional flagellar phase variation of H：z66 positive Salmonella enterica serover Typhi.