中文摘要：

菜蛾盘绒茧蜂Cotesia vestalis（Haliday）是小菜蛾的重要幼虫内寄生蜂，该蜂在胚胎发育过程中由浆膜产生畸形细胞，随蜂卵孵化，释放到寄主小菜蛾的血腔中。本文运用蛋白质双向电泳技术、电子显微技术和体外培养技术对菜蛾盘绒茧蜂畸形细胞的蛋白质的合成和分泌以及细胞超微形态结构的变化进行了研究。蛋白质双向电泳图谱显示，该细胞内蛋白合成种类极为丰富，尤以分子量40．98—94．64kDa的蛋白种类最多。但在寄生后期，畸形细胞的合成能力下降。超微形态结构发生显著变化表现在胞内细胞器数量减少，出现大量空腔，细胞表面微绒毛联合、变大，内容物外倾。随着蜂幼虫的啮出，有些细胞经历分解过程。体外培养证实，成熟畸形细胞可向培养介质中释放脂溶性物质。此外，在不同饲养温度条件下，畸形细胞伴随蜂幼虫的发育，表现为随温度升高，发育加快的趋势，表明畸形细胞的生长趋势与寄生蜂幼虫发育具有同步性。

英文摘要：

The braconid wasp Cotesia vestalis （Haliday） is a primary larval endoparasitoid of the diamondback moth, Plutella xylostella （L.）. Upon egg hatching, many teratocytes originating from the serosal membrane were released into the parasititized larval hemocoele. The present paper was focused on studying the synthesis of protein, secretion and ultrastructural changes of C. vestalis teratocytes by using the related techniques. Protein two-dimensional electrophoresis strong ability of protein synthesis, the proteins with showed that the mature teratocytes of C. vestalis had molecular sizes between 40. 98 to 94. 64 kDa were abundant. During the later stages of parasitism, the ability of protein synthesis in teratocytes declined, and the significant ultrastructural alterations of teratocytes were observed. There were sparse mitochondria and endoplasmia reticulum, and many vacuoles in cytoplasm, coalescence of microvilli on the cell surface, cellular materials discharged. Following the parasitoid＇s emergence, some cells underwent decomposition. In vitro, the mature cells could secrete lipids into medium. At different temperatures, the teratocytes developed quickly accompanying with the development of parasitoid larvae when the temperature increased, it showed that the pattern of teratocytes grew synchronously with the development of parasitoid larvae.