中文摘要：

目的探讨6Gy 137Cs γ射线一次性全身照射后，小鼠血清中白细胞介素10（IL-10）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF—α）水平的变化及对脂多糖刺激反应性的远期影响。方法将实验小鼠分为假照射组和照射组，假照射组不接受照射，照射组给予6Gy1射线照射。照射后10周，将假照射组和照射组小鼠按组别分别提前24h和1h腹腔注射脂多糖（20mg／kg），对照组注射生理盐水（100μl／只）。取小鼠外周血，利用酶联免疫吸附技术检测小鼠血清中IL-10、IL-6和TNF-α的水平。结果假照射组小鼠在给予脂多糖刺激1h后，血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高（t=7.31、2．71和15．09，P分别为〈0．01、〈0．05和〈0．01）。照射组小鼠在给予脂多糖刺激1h后，血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高（f=4．14、7．18和5．14，P均〈0．01）。与假照射组相比，照射组小鼠在给予脂多糖刺激24h后，TNF．仅和IL-6水平无明显变化，IL-10水平升高了19．9％（t=2．84，P〈0．05）。结论经6Gy γ射线照射后10周，小鼠免疫调节功能尚未完全恢复；在接受脂多糖刺激后，假照射组和照射组小鼠的抗感染能力也有差异。脂多糖对辐射后小鼠血清中IL-10、IL-6和TNF-α水平的影响及其意义仍需进一步研究。

英文摘要：

[Abstract] Objective To investigate the effects of lipopolysaccharide （LPS） on interleukin-10（IL- 10）, interleukin-6（IL-6） and tumor necrosis factor-c~（TNF-ct） levels in mice exposed whole body to 6 Gy ~/-ray radiation once. Methods The mice were divided into sham-irradiation group and irradiation group. Mice in sham-irradiation group accepted no irradiation, while mice in irradiation group accepted 6 Gy whole body irradiation at dose rate of 0.8 Gy/min. Mice in LPS treated group accepted intraperitoneal injection of LPS （20 mg/kg） at 10 weeks after irradiation, while mice in control group were accepted intraperitoneal injection of saline. The peripheral blood was collected by eyeballs enucleation after 1 h and 24 h respectively, then IL- 10, IL-6 and TNF-α levels were detected by enzyme-linked immunoassay. Results Compared to control group, the levels of IL-IO, IL-6 and TNF-α in blood serum increased at 1 h and 24 h after LPS injection both in sham-irradiation group （t=7.31, P〈0.01; t=2.71, P〈0.05; t=15.09, P〈0.01 ） and irradiation group 0=4.14, 7.18 and 5.14, all P〈O.01 ）. Compared to sham-irradiation group, the level of IL-10 increased 19.9% at 24 h after LPS injection in irradiation group（t=2.84, P〈0.05 ）, while the levels of TNF-α and IL-6 had no signifi- cant differences between sham-irradiation group and irradiation group. Conclusions The immune system of mice had not entirely recovered at 10 weeks after 6 Gy γ-ray irradiation. After LPS injection, there was difference of anti-infection ability between sham-irradiation group and irradiation group. The effects of LPS on cytokines IL-10, IL-6 and TNF-α levels in radiation-exposed mice need to be further explored.