中文摘要：

目的：研究木犀草素抑制肝纤维化进程中肝细胞上皮间质转化的作用。方法：大鼠腹腔注射50％CCl。橄榄油溶液（1ml／kg），每周2次，连续12周以复制大鼠肝纤维化模型。30只SD大鼠随机均分为正常对照（等容含5％CMC-Na溶液）组、模型（等容含5％CMC-Na溶液）组、木犀草素（100mg／kg）组。第1次腹腔注射CCI。后灌胃给药，每天1次，连续12周。HE和VG染色检测大鼠肝组织病理学变化，免疫组化法检测肝组织Ⅱ一平滑肌肌动蛋白（α-SMA）、转化生长因子（TGF）-β1、波形蛋白（Vimentin）、上皮型E．钙黏蛋白（E-cadberin）的表达。采用门静脉插管Ⅳ型胶原酶原位灌注法分离大鼠原代肝细胞。实验随机均分为空白对照（等容含10％FBS的DMEM溶液）组、模型（等容含10％FBS的DMEM溶液）组与木犀草素高、中、低浓度（40、20、10mmol／L）组。采用实时荧光定量聚合酶链反应法检测α-SMA、I型胶原（CollagenI）、Ⅲ型胶原（CollagenⅢ）、E-cadherin、转录因子Snail、白蛋白（ALB）、糖原合成酶（GS）mRNA的表达；Westernblot法检测α-SMA、Vimentin、E-cadherin蛋白的表达。结果：与模型组比较，木犀草素组大鼠肝组织α-SMA、TGF—β1、Vimentin表达减弱，E-cadherin表达增强，肝脏病理学程度减轻；木犀草素高、中、低浓度组大鼠肝组织细胞α-MA、CollagenI、CollagenⅢ、SnailmRNA表达减弱，木犀草素高、中浓度组大鼠肝组织细胞E-cadherin、ALB、GSmRNA表达增强；木犀草素高、中、低浓度组大鼠肝组织细胞α-SMA、Vimentin蛋白表达减弱，E-cadherin蛋白表达增强。结论：木犀草素具有明显抗肝纤维化的作用，其机制可能与木犀草素抑制肝细胞上皮间质转化作用有关。

英文摘要：

OBJECTIVE： To study inhibitory effect of luteolin on epithelial-mesenchymal transition （EMT） of hepatocytes in rats with hepatic fibrosis. METHODS： Rat liver fibrosis model was induced by subcutaneous injection of 50% CCh 1 ml/kg sc twice a week for consecutive 12 weeks. Luteolin was administered intragastrieally with daily dose of 100 mg/kg once a day for con- secutive 12 weeks after the first injection of CCh. 30 SD rats were randomly divided into solvent control group （5%CMC-Na）, model group （5%CMC-Na） and luteolin group （100 mg/kg）. The pathological changes of liver tissue were observed by HE and VG staining. The expressions of α-SMA, TGF-β1, vimentin and E-cadherin in liver tissues were detected with immunohistochemis- try. In vitro, rat primary hepatocytes were isolated by portal vein intubation and type Ⅳ collagenase in situ perfusion method. They were divided into normal control group （blank culture solution）, model group （blank culture solution） and luteolin high-dose, me- dium-dose and low-dose groups （40, 20, 10 mmol/L）. The expressions of αSMA, Collagen Ⅰ , Collagen Ⅲ, E-cadherin, Snail, ALB, GS gene were detected by real-time RT-PCR. α-SMA, vimentin and E-cadherin protein were detected by western blot. RE- SULTS： Compared with model group, the expressions of α-SMA, TGF-β1 and vimentin in liver tissue were decreased in luteolin group, while the expression of E-cadherin was increased; ;pathological changes of liver tissue were relieved. Compared with model group, the expressions of α-SMA,Collagen I ,Collagen m and Snail gene in liver tissue were decreased in luteolin high-dose, me- dium-dose and low-dose groups, while the expression of E-cadherin,ALB ,GS mRNA was increased gene in luteolin high-concentrα tion, medium-concentration groups, the expressions of α-SMA, Vimentin protein in liver tissue were decreased in luteolin high-dose, medium-dose and low-dose groups, while E-cadherin protein was increased.CONCLUSIONS： Luteolin significantly at- tenuate