Q/q 基因,也作为 WAP2 知道,是为小麦驯服的重要基因并且是抄写因素的 AP2 (APETALA2 ) 班的一个成员。在现在的学习,我们首先孤立 W t AP2 等位基因(在哪儿“ t ”指的上标 speciese 来源,在这种情况中“ tauschii ”) 在从 Aegilops tauschii Coss 的染色体 5D 上,普通小麦的 D 染色体施主种类。我们发现那 W t 从 Arabidopsis 的 AP2 和 AP2 基因分享 AP2 多肽,一个高度基本的 10-amino 酸领域,和一个 AASSGF 盒子的一个中央核心,尽管在 37-amino 酸有许多差别充满丝氨酸的酸的领域和留下的区域。另外, W t AP2 对在核苷酸和氨基酸水平的小麦的 5A 和 5B 上的 homoeologous loci 高度相应。然而,有一些变化,可能与基因功能有关。在第一个 AP2 领域, 5D 和 5A loci 上的氨基酸 VYL 在 5B 上被 LLR 代替。在 37-amino 酸充满丝氨酸的酸的领域, W t 5D 上的 AP2 有额外的氨基酸插入。也在 329 氨基酸位置有一个变化,它被认为与免费脱粒的小麦的外观有关。在这个位置,氨基酸为 q 等位基因是为 Q 等位基因和缬氨酸的 5A 上的异白氨酸,而氨基酸是 5D 和 5B 上的白氨酸。而且,一个偷乘者缩小的终端转换了重复元素(螨) 插入在所有普通小麦和部分 tetraploid Triticum 的 5B 上的 WAP2 的第九 intron 是在场的浮肿嗯小麦。这些结果为研究提供新线索进 WAP2 和普通小麦的起源的进化生物学。
The Q/q gene, also known as WAP2, is an important gene for wheat domestication and is a member of the AP2 (APETALA2) class of transcription factors. In the present study, we first isolated the WRAP2 allele (where the superscript "t" refers to the speciese source, in this case "tauschii") on chromosome 5D from Aegilops tauschii Coss., the D-genome donor species of common wheat. We found that WRAP2 and the AP2 gene from Arabidopsis share a central core of the AP2 polypeptide, a highly basic 10-amino acid domain, and an AASSGF box, although there are many differences in the 37-amino acid serine-rich acidic domain and the remaining regions. In addition, WRAP2 was highly homologous to the homoeologous loci on 5A and 5B of wheat at both the nucleotide and amino acid level. However, there were some variations that are probably related to gene function. In the first AP2 domain, the amino acids VYL on the 5D and 5A loci were replaced with LLR on 5B. In the 37-amino acid serine-rich acidic domain, WRAP2 on 5D had an extra amino acid insertion. There was also a variation at the 329 amino acid position, which is thought to be related to the appearance of free-threshing wheat. At this position, the amino acid is isoleucine on 5A for the Q allele and valine for the q allele, whereas the amino acid is leucine on 5D and 5B. Furthermore, a Stowaway miniature terminal inverted repeat element (MITE) insertion was present in the ninth intron of WAP2 on 5B of all common wheats and partial tetraploid Triticum turgidum wheats. These results provide new clues for studies into the evolutionary biology of WAP2 and the origin of common wheat.