这研究试图孤立并且屏蔽 black-spot-resistant endophytic 真菌紧张。健康罗莎 chinensis 的各种各样的种类在 Shaanxi 省从西安, Xianyang, Baoji 和 Weinan 城市被收集。Endophytic 真菌从茎和叶子被孤立,并且到接种的 2-3 时间净化了与打方法修改屏蔽对 Marssonina rosae 对抗的 endophytic 真菌。从 Xianyang 收集的样品展出了最高的殖民率和隔离率;从茎孤立的 endophytic 真菌紧张与叶子相比介绍了最高的殖民率和隔离率。67 endophytic 真菌紧张的一个总数从罗莎 chinensis 被孤立,包括都从 Baoji 被导出的 3 黑点抵抗的紧张。这研究为进一步屏蔽 biocontrol 真菌和环境友好的真菌的生物控制的候选人紧张打了基础。
[Objective] This study aimed to isolate and screen black-spot-resistant en- dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col- lected from Xi'an, Xianyang, Baoji and Weinan City in Shaanxi Province. Endophytic fungi was isolated from stems and leaves, and purified by 2-3 times of inoculation to screen endophytic fungi antagonistic to Marssonina rosae with modified punching method. [Result] Samples collected from Xianyang exhibited the highest colonization rate and isolation rate; endophytic fungi strains isolated from stems presented the highest colonization rate and isolation rate compared with leaves. A total of 67 en- dophytic fungi strains were isolated from Rosa chinensis, including 3 black spot-re- sistant strains which were all derived from Baoji. [Conclusion] This study laid the foundation for further screening candidate strains of biocontrol fungi and environ- ment-friendly fungal biological control.