中文摘要：

目的观察肠易激综合征（irritable bowel syndrome,IBS）大鼠腹直肌肌电变化以及脊髓背角中NMDAR（N-methyldaspar-tate receportes,NMDAR）、CGRP（calcitonin gene-related peptide）,以及骶髓后联合核（dorsal commissural nucleus,DCN）中小胶质细胞的变化,为研究IBS内脏敏化提供理论依据。方法以旋毛虫感染大鼠致肠易激综合征大鼠模型,然后随机分为2组：IBS无刺激组和IBS结肠刺激组。另外选择5只正常大鼠作为对照。分别测定各组腹直肌肌电以及NMDAR、CGRP表达以及小胶质细胞的变化。结果 IBS结肠刺激组大鼠腹直肌肌电、大鼠骶髓后角中NMDAR和CGRP的表达以及骶髓后联合核中的小胶质细胞活化较正常对照组及IBS未刺激组均显著增强。结论 IBS大鼠内脏敏化可能与骶髓后角神经活性物质活化以及DCN中小胶质细胞的活化相关,这些物质以及细胞的活化进而导致神经元敏化的发生。

英文摘要：

Objective To investigate the myoelectric changes of the rectus abdominis in irritable bowel syndrome（IBS） rats,the expression of NMDAR and CGRP in the dorsal horn of spinal cord of rats with IBS,and the activation of microglial cells in dorsal commissural nucleus（DCN）,and to provide a theoretical basis for visceral hyperalgesia of the rats with IBS.Methods The rats were gavaged with the Trichinella spiralis to establish the irritable bowel syndrome model.The model rats were randomly divided into IBS group and IBS＋colon distension group.Five normal rats were chosen as controls.The myoelectric changes of the rectus abdominis were observed,and the expression of NMDAR,CGRP and changes of microglia were determined using immunofluorescence method.Results The electro-activity of the rectus abdominis,the expression of NMDAR,CGRP and activation of microglia of the sacral joint nuclear were significantly enhanced in IBS rats with colon distension than that in the normal rats and non-stimulation IBS rats.Conclusion The visceral hyperalgesia of IBS may be related with some neuroactive substances and activation of microglia cells in DCN,which will cause the neuronal sensitization.