中文摘要：

目的建立同时测定裸花紫珠中5个有效成分的超高效液相色谱（UPLC）方法。方法采用AgilentEcliDseXDB—C18色谱柱（100mm×3．0mm，1．8μm），流动相为乙腈（A）-0．1％甲酸水溶液（B），梯度洗脱，体积流量为0．7mL／min。检测波长350nm，柱温40℃。结果木犀草苷、毛蕊花糖苷、木犀草素-4’-O-β-D-吡喃葡萄糖苷、木犀草素、5，4＇-二羟基-3．7．3＇-三甲氧基黄酮分别在2．44～122．0μg／mL（r=0．9998）、9．06-453．0μg／mL（r=O．9999）、4．42-221．0μg／mL（r=0．9999）、3．36～168．0μg／mL（r=0．9998）、2．52～126．0μg／mL（r=0．9998）内线性关系良好；平均回收率在98．5％～100．8％。结论所建立的UPLC法简便快速、重复性良好、结果准确可靠，可作为裸花紫珠药材质量标准控制的方法。

英文摘要：

Objective To develop a UPLC method for the stimultaneous determination of five active ingredients in Callicarpa nudiflora. Methods Analysis was performed on an Agilent Eclipse XDB-C18 column （100 mm ×3.0 mm, 1.8 μm） eluted with acetonitrile （A） and 0.1% methanoic acid （B） in a gradient program. The flow rate was 0.7 mL/min, the detection wavelength was 350 nm, and the column temperature was 40 ℃. Results Luteoloside, acteoside, luteolin-4＇-O-β-D-glucopyranoside, luteolin, and 5, 4＇-dihydroxy-3, 7, 3＇-trimethoxyflavone showed a good linearity in the ranges of 2.44--122.0 μg/mL （r = 0.999 8）, 9.06-453.0 μg/mL （r = 0.999 9）, 4.42--221.0 μg/mL （r = 0.999 9）, 3.36-- 168.0μg/mL （r = 0.999 8）, and 2.52-- 126.0 μg/mL （r = 0.999 8）. The average recoveries, measured at three concentration levels, varied from 98.5%-- 100.8%. Conclusion The method is simple, accurate, and can be used for the quality control of C. nudiflora.