中文摘要：

选取在水产养殖过程中广泛使用的磺胺类抗生素磺胺嘧啶对斑马鱼胚胎进行毒性试验，依据水环境磺胺类药物的检出水平，研究了低质量浓度（0.001 mg/L、0.1 mg/L、10 mg/L）磺胺嘧啶对斑马鱼早期发育的毒性效应。在磺胺嘧啶暴露过程中，以孵化的斑马鱼胚胎为试验模型，设置空白对照组和药物组，观察记录孵化率、死亡率、发育畸形、心率和自主运动次数。在标准条件下将斑马鱼胚胎置于培养皿，对其进行96 hpf的磺胺嘧啶暴露处理，分别在第8 h、24 h、48 h、96 h观察所得的毒理学终点。结果表明，低质量浓度的磺胺嘧啶对斑马鱼胚胎的孵化、心率和自主运动产生了不利的影响，且孵化率、致死率、致畸率、心率和自主运动等指标呈现剂量依赖性。低质量浓度的磺胺嘧啶暴露，可显著促进斑马鱼的自主运动（ p 〈0.001），增大斑马鱼的心率（ p 〈0.001），未见斑马鱼心脏组织异常。斑马鱼胚胎在药物暴露处理过程中，均产生畸形效应，主要表现为出血、凝血、尾部弯曲、心包水肿和鳔缺失。这些现象表明，水体中抗生素残留存在潜在生态风险，抗生素的生态风险问题不容忽视，并证明了利用斑马鱼建立简便、准确、直观、快速的检测模型和检测方法的可行性。

英文摘要：

The present paper takes it as its research topic to evaluate the potential risk for the fish embryos by investigating the use of sulfadiazine as the toxic agent. In doing so, the zebrafish embryos were let to be exposed at low concentrations （0.001 mg/L, 0.1 mg/L） of sulfadiazine for the study of toxicity on the growth of the said fish in accordance with the detectable concentration of sulfonamides in the water environment. Later, the bio-toxicity of the low concentration sulfadiazine antibacterial has been investigated by observing the lethal and sub-lethal effects on the zebrafish （ Danio rerio ） embryos and larvae. More specifically speaking, the newly fertilized zebrafish eggs were exposed under the static conditions in a culture dish for 4 days to the pharmaceutical sulfadiazine by dividing the fertilized zebrafish eggs randomly into the control groups with different concentrations of the drug groups based on the concrete experiments. Then, the investigation has been done for the hatching rate of the said fertilized zebrafish eggs, and their mortality, non-lethal malformation, heart rate, spontaneous movement, etc., to be observed and recorded in detail at the specified stages. At the same time, the endpoints were recorded at 8 h, 24 h, 48 h and 96 h post fertilization （hpf）, including the mortality, malformations and other sub-lethal responses. The results of our investigation reveal that the low concentration sulfadiazine had adverse impact on the hatch, heart rate and spontaneous movement, with the indexes such as hatching rate, fatality rate, teratogenic rate and spontaneous movement being analyzed in dose-dependent manner. The low concentration of sulfadiazine has been found able to stimulate the spontaneous movement of the zebrafish （ p 〈0.001） and speed up the heart movement rate of the fish noticeably （ p 〈0.001） in comparison to the control group. However, during the entire period of the exposure, no harm was found to the heart tissue activities. However, something has happen