中文摘要：

目的分析细粒棘球蚴感染小鼠单核髓源抑制性细胞（M-MDSC）精氨酸酶的表达和活性变化。方法 12只BALB/c雌性小鼠随机分为对照组和感染组。感染组小鼠腹腔注射活原头节2 000个/只,对照组小鼠注射等体积生理盐水。于感染后120 d采集小鼠眼眶静脉丛外周血,解剖并观察小鼠腹腔和各脏器的病变。无菌取小鼠脾组织,制备单细胞悬液后,利用免疫磁珠分离M-MDSC。提取并纯化M-MDSC RNA,合成c DNA,芯片检测筛选感染组和对照组的M-MDSC差异基因,荧光定量PCR验证差异基因的表达。精氨酸酶检测试剂盒检测小鼠外周血中的精氨酸酶活性。结果BALB/c小鼠感染细粒棘球蚴原头节后120 d,腹腔和内脏器官中形成单性包囊。免疫磁珠分离获得M-MDSC。芯片杂交和荧光定量PCR检测结果显示,感染组小鼠M-MDSC源精氨酸酶相对表达量分别为7.92±0.85和11.97±5.39,均高于对照组小鼠（1.65±0.19和1.00±0.57）（P〈0.05）。检测结果表明,感染组小鼠外周血中的精氨酸酶活性为（3.83±0.44）U/L,高于对照组小鼠[（1.57±0.57）U/L]（P〈0.05）。结论细粒棘球蚴感染小鼠M-MDSC精氨酸酶的表达量和活性显著升高。

英文摘要：

Objective To investigate the alteration of expression and activity of arginase from monocytic-type myeloid-derived suppressor cells（M-MDSC） in BALB/c mice infected with Echinococcus granulosus. Methods Twelve BALB/c female mice were randomly divided into control and infected groups. The mice were injected in-traperitoneally with 2 000 live protoscoleces or an equivalent volume of normal saline. After 120 days, peripheral blood was collected through venae orbitaeta, and mice were sacrificed for pathological examination. The spleen was collected under aseptic conditions and single-cell suspension was prepared for M-MDSC isolation using the magnetic bead separation technology. Total RNA was extracted from M-MDSC, c DNA was generated, and genes with differential expression without and with infection were screened using the chip hybridization method. The resulting genes were further validated using real-time PCR. The activity of arginase from peripheral blood was also measured. Results Single cyst was formed within the abdomen and internal organs 120 days after infection. Chip hybridization and real-time PCR showed that the relative expression of arginase from M-MDSC in the infected group（7.92 ±0.85 and 11.97 ±5.39, respectively） was significantly higher than that in the control group（1.65 ±0.19 and 1.00 ±0.57,respectively）（P〈0.05）. The activity of arginase was also significantly higher in the infected group [（3.83±0.44）U/L ]than in the control [（1.57±0.57） U/L]. Conclusion The expression and activity of arginase from mouse M-MDSC both increase significantly after infection with Echinococcus granulosus.