中文摘要：

目的观察基质细胞衍生因子-1（stromal-derivedfactor-1,SDF-1）在鸡卵清蛋白（OVA）诱导小鼠哮喘模型中的表达,探索哮喘气道重塑的发生机制。方法 40只雌性SPF级C57BL/6小鼠,体重18～22 g,随机分为哮喘组和对照组。按照文献方法建立OVA小鼠哮喘模型。两组动物于末次雾化结束后24 h处死,取肺组织行病理切片,苏木精-伊红染色（HE）观察肺脏的组织病理学变化;逆转录聚合酶链式反应（RT-PCR）、免疫印迹法（Western blot）、免疫组织化学（immunohistochemistry,I H）技术检测肺组织SDF-1的表达。结果①哮喘组支气管上皮细胞脱落、气道壁增厚,细支气管和血管周围有大量炎细胞浸润;②哮喘组肺组织SDF-1在RT-PCR、Western blot及I H中均为阳性表达,而对照组则否。结论小鼠哮喘时肺组织分泌大量细胞趋化因子SDF-1,可能是哮喘气道重塑的重要因素之一。

英文摘要：

Objective To study the expression of stromal-derived factor-1（SDF-1） in the airway of mice with allergic asthma induced by ovalbumin（OVA） so as to explore the mechanism of asthmatic airway remodeling.Methods Forty female C57BL/6 mice of SPF grade were randomly divided into asthma group and control group with 20 in each.Mice were sensitized and challenged with ovalbumin to establish the asthmatic model.All the mice were killed 24 hours after the last aerosolisation.The paraffin embedded sections of lung tissues were stained with hematoxylin and eosin（HE） for analyzing pathological changes.SDF-1 mRNA extracted from lung tissues was assessed by RT-PCR while SDF-1 protein extracted from lung tissues was assessed by Western blot and immunohistochemistry（IH）.Results ① After repeated allergen challenge,obvious infiltration of inflammatory cells and proliferation of goblet cells and smooth muscles appeared in mouse bronchi.② According to RT-PCR and Western blot and IH results,the expression of SDF-1 was positive in asthma group but negative in control group.Conclusion A large amount of SDF-1 is secreted in the lung tissues in asthmatic mice,which may be one of the critical factors in asthmatic airway remodeling.