中文摘要：

目的探讨白花蛇舌草对人骨肉瘤MG-63细胞Bax基因表达的影响。方法采用MTT法检测一定浓度白花蛇舌草注射液对MG-63细胞作用6、12、24、48h后的细胞增殖抑制率,实时荧光PCR（RT-PCR）检测细胞内Bax基因的表达情况。结果白花蛇舌草注射液能明显抑制MG-63细胞增殖,浓度为100μL/mL时,随着作用时间的延长,Bax基因表达明显升高（P〈0.05）。结论白花蛇舌草注射液可能通过上调Bax基因表达诱导人骨肉瘤MG-63细胞凋亡。

英文摘要：

Objective To investigate the effect of hedyotic diffusa willd injection on osteosarcoma MG-63 cells Bax gene expression.Methods MTT was be used to detect the inhibition rate of MG-63 cells by hedyotic diffusa willd injection after 6,12,24,48 h certain concentration,RT-PCR was be used to detect the intracellular expression of Bax gene.Results Hedyotic diffusa willd injection can significantly inhibit the proliferation of MG-63 cells,as the concentration was 100μL/mL,Bax gene expression was significantly increased over time（P0.05）.Conclusion Upregulating the expression of Bax gene by hedyotic diffusa willd injection can induced human osteosarcoma MG-63 cells apoptosis to death.