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中文摘要：
目的探讨创伤后应激障碍（PTSD）大鼠蓝斑神经元β-catenin（β-连环蛋白）的表达变化。方法采用国际认定的SPS方法刺激建立大鼠PTSD模型,取成年健康雄性Wistar大鼠100只,随机分为连续单一刺激（single prolongedstress,SPS）模型1d、4d、7d、14d组和对照组,应用免疫组化、免疫印迹方法检测PTSD大鼠蓝斑神经元β-catenin的表达变化;透射电镜观察PTSD大鼠蓝斑神经元的超微结构变化。结果经SPS刺激后大鼠蓝斑神经元细胞内β-catenin于1d开始逐渐减少,14d表达最少;蓝斑神经元出现细胞凋亡改变。结论蓝斑神经元细胞凋亡可能是导致PTSD患者蓝斑功能失调的重要原因之一。
英文摘要：
Objective To investigate the expression of β-catenin in the locus coeruleus of rats with post-traumatic stress disorder （PTSD） . Methods The SPS-method was used to set up the rat PTSD models. A total of 100 health , male Wistar rats were randomly divided into 1, 4, 7 and 14d groups of single prolonged stress （ SPS ） and a normal control group. Immunohistochemistry , immunofluorescence,and western blot were used to observe and detect the changes in the expression of β-catenin in the locus coeruleus neurons, while ultrastructure was detected by electron microscopy. Results Apoptotic cells were present in the locus coeruleus of PTSD rats. The expression of β-catenin in the locus coeruleus gradually decreased from ld after SPS, and reached the minimum at SPS 14d. Conclusion The changes of β-catenin expression in the locus coeruleus may play important roles in the dysfunction of the locus coeruleus in PTSD patients
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