[目的]建立瑶药红凉伞的质量分析方法。[方法]采用TLC法鉴别红凉伞药材的主要成分,根据2010年版《中华人民共和国药典》的方法对红凉伞药材的水分、总灰分及醇溶性浸出物进行测定。[结果]本实验的薄层色谱条件有较好的分离效果,且重复性较好,薄层色谱鉴别特征可以作为红凉伞药材的鉴别依据;水分、总灰分及醇溶性浸出物的测定结果为:水分8.84%~12.40%,总灰分2.62%~4.80%,酸不溶性灰分0.37%~0.97%,浸出物含量13.40%~16.85%。[结论]本实验的鉴别方法可用于红凉伞的质量控制,研究结果可为红凉伞的开发利用和建立质量标准提供科学依据。
[Objective] To establish the quality analysis method for Ardisia crenata Sims. var. bicolor (Walker) of Yao medicine. [ Methods ] TLC was performed to identify the main component of A rdisia crenata. The moisture, total ash and alcohol-soluble extract were determined based on the Chinese pharmacopoeia of 2015 edition. [Results] The TLC conditions of this experiment have good separation effect and reproducibility. TLC identification characteristics could be regarded as the distinguishing basis of A rdisia crenata. The moisture, total ash content and alcoholic- soluble extract content were 8.84% ~ 12.40%, 2.62% ~ 4.80%, 0.37% ~0.97% and 13.40% ~ 16.85% respectively. [Conclusion] The identification method of this experiment can be used for the quality control of Ardisia crenata. The research results can provide scientific basis for the development and utilization of Ardisia crenata and establishment of quality standard.