中文摘要：

通过农杆菌介导转化法,将O型口蹄疫病毒P12A^＊3C基因转化到玉米自交系18-599的Ⅱ型胚性愈伤组织中,经潮霉素抗性筛选获得25株抗性再生植株。对转基因植株的PCR检测,PCR-Southern及RT-PCR检测表明,目的基因P12A^＊3C已经整合到玉米基因组中并得到表达。T1代植株的PCR检测表明,P12A^＊3C基因能稳定遗传,本研究为转基因玉米口蹄疫可饲疫苗的生产奠定了试验基础。

英文摘要：

The P12A^＊3C gene of food-and-mouth disease virus（FMDV） type O was transformed into type Ⅱ callus maize inbred line 18-599 via Agrobacterium-mediated transformation.25 resistant transgenic plants were obtained after the primary screening on the medium containing hygromycin.The detection by PCR,PCR-southern and RT-PCR indicated that the P12A^＊3C gene was integrated into the genome of 18-599 and expressed.The detection of T1 generation indicated that the P12A^＊3C gene could be stably transmited to the next generation.These results provided experimental evidence for production of oral FMDV vaccine.