背景词法并且在 Koch 三角的心脏的房间的生理的特征仍然是的电镀物品争论了。我们学习了外观和这些多样的 myocytes 的电的性质阐明他们的复杂电镀物品生理的现象。方法实验用凉下来的充电联合被进行设备(电荷耦合器件) 系统和整个房间,补丁夹钳技术决定形态学,行动潜力和钠当前的密度 ofs 壁炉酶的联盟者从兔子心的 Koch 三角孤立的可行 myocytes。ResultsMorphologically,在蜘蛛,微小的锭子,苗条锭子,杆和长带的形状的心脏的房间在 3.0 ± 0 .3, 35.0 ± 5 .0, 15.0 ± 2 .0, 40.0 ± 5 .0 和 6.0 ± 0 的百分比被观察 .7 分别地。 Thecellular 尺寸和电容逐渐地在上述顺序增加了(所有 P 【 0.05 ) .Electrophysiologically ,从他们记录的行动潜力配置分别地类似于节的(N),在伊朗的银币节(一),节的 Hisian ( NH ),在伊朗的银币,(A)和 Hisian 喜欢从未经触动的心房与心室的节的准备获得的潜力。心脏舒张的去极除了杆房间出现在 allmyocytes。在微小的锭子的目增加的钠当前的密度,长带,杆,苗条锭子房间(所有 P 【 0.05 ) ,但是不能在塑造蜘蛛的细胞被检测。Linearregression 分析表明那个膜电容否定地被相关到心脏舒张的去极 r=-0.70 的率, P 【 0.001,但是断然,到最大的去极,潜力,行动潜力的振幅,向上的一击速度和最大的山峰钠水流 densityr=0.84 珍视, 0.80, 0.87 和 0.75 分别地;所有 P 【 0.001。结果表明了的结论塑造 thatspider,在 Koch 三角力量的锭子,杆和长带房间对应于步调调整,过渡在象房间一样的伊朗的银币和 Purkinje,分别地。而且,微小的锭子和苗条锭子房间被指过渡房间α(TC α) 和β(因此考虑他们的特殊电的性质的 TC β) 。有多样的电的性质的不同 myocytes 组成了复杂电镀物品的基础结构在 Koch 的生理的现象三角。突出的百分比和电的性质,微
Background The morphological and electrophysiological characteristics of cardiac cells in Koch triangle are still disputed. We studied the appearance and electrical properties of these diverse myocytes to elucidate their complex electrophysiological phenomena. Methods Experiments were conducted using cooled charge coupling device (CCD) system and whole cell, patch clamp technique to determine the morphology, action potential and sodium current density of single viable myocytes enzymatically isolated from the Koch triangle of rabbit hearts. Results Morphologically, cardiac cells in shape of spider, tiny spindle, slender spindle, rod and strip were observed in percentage of 3.0±0.3, 35.0±5.0, 15.0±2.0, 40.0±5.0 and 6.0±0.7 respectively. The cellular dimensions and capacitance gradually increased in the above order (all P〈0.05). Electrophysiologically, action potential configurations recorded from them were similar respectively to nodal (N), atrial nodal (AN), nodal Hisian (NH), atrial (A) and Hisian like potentials obtained from the intact atrioventricular nodal preparations. Diastolic depolarization appeared in all myocytes except for rod cells. Sodium current density increased in the order of tiny spindle, strip, rod, slender spindle cell (all P〈0.05), but could not be detected in spider-shaped cells. Linear regression analysis revealed that membrane capacitance was correlated negatively to the rate of diastolic depolarization r=-0.70, P〈0.001, but positively to maximum depolarization potential, amplitude of action potential, upstroke velocity and maximum peak value of sodium current density r=0.84, 0.80, 0.87 and 0.75, respectively; all P〈0.001. Conclusions The results demonstrated that spider-shaped, spindle, rod and strip cells in Koch triangle might correspond to pacemaking, transitional, atrial and Purkinje like cells, respectively. Furthermore, tiny spindle and slender spindle cells were referred to transitional cell α (TCα) and β (TCβ) accordingly consid