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中文摘要：
在pH 4.6的乙酸-乙酸钠缓冲溶液中,环丙沙星能与四苯硼钠作用生成沉淀产生光散射信号（LS）能应用普通荧光分光光度计检测,从而建立了环丙沙星的光散射分析法.LS光谱在385.0 nm处,具有特征散射峰,且增强的光散射强度（ΔILS）与0.03～0.2 μg/mL,0.2～2.5 μg/mL两个范围内的环丙沙星溶液呈线性关系,相对应的检出限分别为7.4,2.7 ng/mL.本实验中研究了酸度、聚乙二醇600溶液的浓度、四苯硼酸钠溶液的浓度以及共存物质对LS强度的影响.方法已用于盐酸环丙沙星片剂中环丙沙星的测定,RSD为0.3%～0.6%.
英文摘要：
In the medium of HAc-NaAc buffer solution （pH 4.6）, a light scattering analytical method for the detemir otion of ciprofloxacin was established based on the interaction of ciprofloxacin with sodium tetraphenylboron, produces precipitation and results in light scattering （IS） signals measured with Fluorescence spectrophotometer and characterized by peak at 385.0 mm. It was found that the enhanced IS intensities （△I15） were in proportion to the concentrations of ciprofloxacin in two ranges. The linear ranges of ciprofloxacin are 0.03 - 0.2 μg/mL and 0.2 - 2.5 μg/mL. The detection limits for ciprofloxacin are 7.4 and 2.7 ng/mL, respectively. The effects of the pH, the concentration of polyethyleneglycol 600 solution, the concentration of sodium tetraphenylboron solution and foreign substances on the determination of ciprofloxacin were investigated. The present method is successfully applied to the determination of ciprofloxacin in ciprofloxacin hydrochloride tablets with the RSD of 0.3% -0.6%.
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