瞄准:为了调查浆液的效果,源于在表皮的生长的表示上在胃顶点 acupoints 在电镀物品针灸方面对待的老鼠在胃的粘膜房间的因素受体(EGFR ) 基因。方法:在老鼠模型的导致压力的胃的粘膜损害被水沉浸建立并且制止了压力方法。52 只老鼠随机被划分成:正常的组(n = 8 ) ,模型组(n = 8 ) ,模型浆液组(n = 12 ) ,胃浆液组(n = 12 ) ,并且胆囊浆液组(n = 12 ) 。胃的粘膜房间被 pronase-EDTA 消化方法分开并且与浆液孵化了。在胃的粘膜房间的 EGFR 基因表示被反向的抄写聚合酶检测链反应(RT-PCR ) 方法。结果:与正常的组相比( 0.6860 +/- 0.0594 ),从胃的老鼠导出的浆液组织( 1.2272 +/- 0.0813 , P = 0.00 【 0.01 )并且胆囊组( 0.9640 +/- 0.0387 , P = 0.00 【 0.01 )有一个趋势在胃的粘膜房间提高 EGFR 基因表示。如此的趋势在模型组存在(0.7104 +/- 0.0457 ) 但是没有有效差量(P = 0.495 】 0.05 ) 并且在模型浆液组(0.8516 +/- 0.0409 ) 与极其明显的差别(P = 0.001 【 0.01 ) 。而且,在胃浆液组的 EGFR 基因表示在胆囊浆液组比那显著地高(P = 0.00 【 0.01 ) 。结论:那浆液从在胃顶点 acupoints 在电镀物品针灸方面对待的老鼠导出的现在的学习表演能清楚地增加胃的粘膜房间的 EGFR 基因表示。因此,在浆液有某些顶点特性,它能为 TCM 理论提供一个证明“在顶点和内部机关之间的特别关系”。
AIM: To investigate the effect of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expression of epidermal growth factor receptor (EGFR) gene in gastric mucosal cells. METHODS: The stress-induced gastric mucosal injury in rat model was established by water-immersion and restrained stress methods. 52 rats were randomly divided into: normal group (n = 8), model group (n = 8), model serum group (n = 12), stomach serum group (n = 12), and gallbladder serum group (n = 12). The gastric mucosal cells were separated by pronase-EDTA digestion method and incubated with serum. The EGFR gene expression in gastric mucosal cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: Compared with normal group (0.6860 ± 0.0594), the serum derived from rats of the stomach group (1.2272 ± 0.0813, P = 0.00 〈 0.01) and gallbladder group (0.9640 ± 0.0387, P = 0.00 〈 0.01) had a tendency to enhance the EGFR gene expression in gastric mucosal cells. Such tendency existed in the model group (0.7104 ± 0.0457) but with no significant difference (P = 0.495 〉 0.05) and in model serum group (0.8516 ± 0.0409) with an extremely obvious difference (P = 0.001 〈 0.01). Furthermore, the EGFR gene expression in stomach serum group was significantly higher than that in gallbladder serum group (P = 0.00 〈 0.01). CONCLUSION: The present study shows that serum derived from rats treated with electroacupuncture at stomach meridian acupoints can distinctly increase the EGFR gene expression of gastric mucosal cells. Therefore, there is certain meridian specificity in the serum, which could provide a proof for the TCM theory "particular relation between meridian and internal organ".