中文摘要：

目的探讨Cd^2＋在Fura-2荧光测定胞质Ca^2＋过程中对荧光的干扰作用.方法培养的PC12细胞,在无钙溶液中利用Fura-2荧光探针测定胞质内Ca2＋浓度.结果在thapsigargin诱导Fura-2荧光比值（F340/F380）上升后,CdC12可以导致F340/F380进一步升高.将细胞膜破坏,Fura-2从胞质内溢出,CdC12依然可以导致荧光比值明显上升.在无PC12细胞,无Ca^2＋的溶液中,加入Fura-2/AM,100 μmol·L^-1 CdC12可以导致F340/F380明显升高.结论 Cd^2＋对Fura-2及Fura-2/AM都存在荧光干扰现象,使荧光比值增加.

英文摘要：

Aim To investigate the blockade of cadmium on store operated calcium channels and the fluorescence interference of cadmium with Fura-2. Methods PC12 ceils were used to determine the intracellular calcium concentration [ Ca^2＋ ] i indicated by change in Fura-2 fluorescence ratio（ F340/F380 ）. Resuits Introduction of cadmium induced a significant increase in Fura-2 fluorescence ratio following thapsigargin-evoked calcium entry; Besides, cadmium gave rise to a remarkable elevation in Fura-2 fluorescence ratio following breakdown of the plasma membrane by triton, a detergent. The Fura-2 fluorescence was increased in the presence of cadmium and Fura-2/AM, simultaneously in the absence of PC12 cells. Conclusion Cadmium can interfere with the Fura-2 fluorescence.