中文摘要：

目的探讨不同蝎毒多肽（scorpion venom peptide，SVP）组分对辐射后机体造血干细胞及祖细胞恢复的作用。方法6．0GyX射线一次性全身照射，制作辐射损伤小鼠模型。内源性脾结节法观察照射后第10天脾集落形成单位（CFU—s）的变化。用甲基纤维素半固体培养基培养骨髓混合集落生成单位（CFU-Mix），观察体内外给药方法及照射后不同时间对CFU-Mix生成的影响。结果（1）体内实验：SVPIV组分处理后的CFU-S数明显高于照射对照组（P〈0．05）；SVPV组分CFU-S数量与照射对照组差异无统计学意义。照射后各SVP组CFU—Mix的数量均高于照射对照组，差异有统计学意义（P〈0．05）。（2）体外实验：与照射对照组相比，体外分别单独加入SVPIV、V组分以及细胞因子（IL-6和SCF）均能够促进CFU-Mix的增殖；而SVPⅣ、Ⅴ组分分别与细胞因子联合应用对CFU—Mix生成的促进作用更为明显，其中Ⅳ组分效果更强，与照射对照组相比差异均有统计学意义（P〈0．05）。结论SVP具有保护辐射损伤小鼠造血干细胞及祖细胞，加速其增殖能力恢复的作用。

英文摘要：

Objective To investigate the effect of different components of scorpion venom peptide （SVP） on stem and progenitor cells in irradiated mice. Methods Mice were exposed to 6.0 Gy X-rays. The number of colony-forming unit of spleen （CFU-S） was calculated at the 10th day after irradiation, and the methylcellulose culture method was used to detect the CFU-Mix on the irradiated mice at different time in vivo and in vitro. Results The study of SVP components in vivo showed that the number of CFU-S was significantly elevated by SVPivafter the irradiation,and CFU-Mix was also increased by both SVPⅣ and SVP Ⅴ. In vitro experiment showed that the number of CFU-Mix was much higher in SVPIV, SVP V and cytokines groups respectively, as compared with control group. Both SVP IV and SVP V had prominent effects in the formation of CFU-Mix when combined with cytokines. Conclusion SVP has a protective effect on the bone marrow hematopoietic stem and progenitor cells in irradiated mice.