中文摘要：

拟南芥钙调素结合蛋白IQM家族共有6个成员,已证实IQM1是一个不依赖Ca2＋的钙调素结合蛋白,其功能缺失突变体iqm1表现气孔开度小和根短的表型,而且突变体气孔开度并不因光、暗、脱落酸等诱导而变大或变小。该实验构建了IQM1基因双元表达载体并转化拟南芥,通过分子筛选及IQM1表达量分析,获得了IQM1基因过量表达植株。表型分析发现,IQM1过量表达植株在光诱导气孔开放处理后气孔开放度明显比野生型和iqm1-1增大,在暗诱导气孔关闭处理后气孔开度则显著变小;IQM1过量表达植株的主根比野生型和iqm1-1长,侧根数量比野生型和iqm1-1多,但IQM1过量表达对植株的生长形态、抽薹期、开花期及座果等方面却没有明显影响。研究表明,IQM1基因在植物气孔运动及根系生长中起着重要作用。

英文摘要：

In Arabidopsis,IQM family has six members and protein. We previously found that the loss of-function mutant iqrnl displayed a smaller stomatal aperture and a shorter primary root,and did not obviously change its stomatal aperture when treated with light, darkness or AlgA. Here, we constructed a binary expression vector harboring the IQM1 gene, and introduced it into wild-type Arabidopsis,and obtained 10 IQM1 overexpression lines through PCR identification and semiquantitative RT-PCR assay. The IQM1 overexpression lines exhibited significantly bigger and smaller stomatal aperture than wild-type and iqml-t when treated with light and darkness, respectively. These lines also showed longer primary root and more lateral root than wild-type and iqml-l. Besides these IQM1 overexpression lines did not display phenotype in plant growth, bolting, flowering and fruiting. These results further suggest that IQM1 plays an important role in stomatal movement and root growth.