中文摘要：

目的探讨维生素C对小鼠丙泊酚麻醉后血清及脑组织抗氧化能力的变化。方法取50只6~8周龄、体质量20~24 g昆明雄性小鼠,随机分为0.9%氯化钠注射液对照组、丙泊酚（propofol,PPF。以下简称P）80 mg/kg组、P 60 mg/kg＋Vc67 mg/kg（简称P60＋Vc67,下同）组、P55＋Vc100组、P50＋Vc200组（n=10）,采用腹腔注射给药的方式,给予小鼠丙泊酚与维生素C不同剂量配比的混合药物,待小鼠翻正反射消失1 min后,进行眼眶后静脉丛采血及提取脑组织,利用分光光度计及酶标仪测定各组血清及脑组织中丙二醛、超氧化物歧化酶的含量及总抗氧化能力。结果 P60＋Vc67组、P55＋Vc100组、P50＋Vc200组中小鼠血清中的丙二醛值均低于P80组;P60＋Vc67组、P55＋Vc100组、P50＋Vc200组中小鼠血清中的超氧化物歧化酶、总抗氧化能力值均高于P80组。P55＋Vc100组及P50＋Vc200组小鼠脑组织中的丙二醛值均低于P80组;P60＋Vc67组、55＋Vc100组及P50＋Vc200组小鼠脑组织中的超氧化物歧化酶、总抗氧化能力值均高于P80组。结论维生素C可使昆明鼠丙泊酚麻醉后血清及脑组织总抗氧化能力提高。

英文摘要：

Objective To explore the antioxidant capacity of propofol combined vitamin C by observing the change of level of MDA, SOD, T-AOC in serum and brain tissue of KM mice. Methods Fifty male KM mice with age of 6-8 weeks and weight of 20-24 kg were randomly divided into five groups： 0.9% sodium chloride injection control group, 80 mg/kg propofol（PPF） group, 67 mg/kg P60＋Vc group, 100 mg/kg P55＋Vc group and 200 mg/kg P50＋Vc group, 10 in each group. Rats were injected intraperitoneally with different dose of mixture of propofol and vitamin C, and after 1 min of loss of righting reflex（LORR）, blood sample from orbital venous and brain tissues were collected, and MDA, SOD, T-AOC in serum and brain of all groups were measured by ELISA. ResultsCompared with PPF80 group, the MDA level of serum in P60＋Vc67, P55＋Vc100, P50＋Vc200 group was lower（P 〈0.05）, while the SOD, T-AOC level of serum in P60＋Vc67, P55＋Vc100, P50＋Vc200 group was higher（P 〈0.05）. And the MDA level of serum in P55＋Vc100, P50＋Vc200 group was lower（P 〈0.05）, the SOD, T-AOC level of serum of P60＋Vc67, P55＋Vc100, P50＋Vc200 group was higher（P 〈0.05）. Conclusion Vitamin C can enhance the total antioxidant capacity after KM mice undertaking propofol, which indicates that vitamin C can increase the antioxidant capacity of KM mice after propofol anesthesia.