采用溶液培养的方法,比较了缺氮和充足供氮条件下燕麦根组织DNA提取液的电泳图谱以及根系分泌物中有机酸、游离氨基酸、可溶性糖含量的差别。缺氮燕麦根基部组织梯状DNA的出现证实缺氮条件下燕麦根轴皮层细胞发生了程序性死亡,过去发现的缺N或缺植物根轴内的空腔或通气组织即是细胞程序性死亡的结果,而且在缺氮胁迫下,根基部细胞首先发生程序性死亡。燕麦根系分泌物的分析结果表明,根系的游离氨基酸和可溶性糖数量与根细胞的PCD无关,但缺氮燕麦根系总有机酸的分泌量显著大于供氮处理的结果意味着缺氮燕麦根轴解体细胞的大分子物质有可能以有机酸的形式释放。
Oat roor tissue DNA electrophoresis, and organic acids, amino acids, soluble sugar in root exudations were compared between different N levels by using solution culture. DNA ladder appeared in proximal ends of oat roots suugested that programmed cell death happened in oat root with no N supply. Therefore the arenchyma or cavum formed in root cortex under N or P deficiencies can be deduced as results of cortical cell PCD. Additionally, the result of this research also suggested the PCD initiates in proximal part of root under N dificiency.The results that amino acids and soluble sugar concentrations in oat root exdudation under no N condition were not significantly higher than those under abundant N supplies suggested that amino acids and soluble sugar concentrations in oat root exdudation has nothing to do with root PCD. However, the reasult that more organic acids were exudated by oat roots with no N than those with N implied that organic acids exudated by oat root may relate to the PCD happened in root cortex.