选用两个优质小麦品种烟农15号和济麦19号,研究了施氮水平对小麦子粒蛋白质组分和加工品质的影响。田间试验设4个施氮水平,即N0、120、240和360kg/hm^2。结果表明,施用氮肥对子粒发育前期清蛋白和球蛋白含量有明显的提高效应,但随子粒灌浆充实,这种效应逐渐削弱,到成熟期,施氮处理虽能提高子粒清蛋白和球蛋白的含量,但不同施氨水平间无明显差异。施用氮肥还能显著地提高子粒醇溶蛋白和麦谷蛋白的含量,尤其是子粒麦谷蛋白的含量,使子粒麦谷蛋白/醇溶蛋白比值提高。试验还表明,施用氮肥能明显提高子粒湿面筋含量,延长面团形成时间、面团稳定时间和断裂时间。综合分析看出,子粒醇溶蛋白和麦谷蛋白的含量以及麦谷蛋白/醇溶蛋白比值是影响小麦加工品质的重要因素,可以作为小麦品质育种中亲本及后代材料的选择、评价和优质栽培技术评价的依据。
A field experiment was conducted to study the effects of nitrogen application rate on the protein components and processing quality of wheat grain using two high-quality cuhivars, Yannong 15(YN15) and Jimai 19 (JM19). Four nitrogen application rates, 0 (CK) ,120 (LO) ,240 (MD) and 360 (HI) kg/hm^2 of N (urea) were used, and field plots were arranged in a randomized complete block design with three replications. Results showed that N fertilization markedly increased both albumin and globulin contents at early period of grain fiUing, but this response was weakened stepwise afterward. Till the maturity, there was no significant difference in albumin and globulin content under different N levels. N application could significantly increase both gliadin and glutenin, especially the latter, consequently the ratio of glutenin to gliadin content increased. N application could also increased wet gluten content of flour and prolonged dough development time, stability time and breakdown time. Comprehensive analysis indicated that both the content of gliadin and glutenin and the gliadin/glutenin ratio were important factors determining the processing quality of wheat grain, and it could be used as the indexes to evaluate the parents-offspring selection in wheat quality breeding and cultivation techniques in wheat production.