为了探知单穗重增加的动态遗传机制,利用由穗型差异大的水稻品种密阳46和FJCD建立的一个包含130个株系的F。重组自交系,测定其在福建武夷山和莆田环境下灌浆期单穗重的动态变化,并进行QTL定位及其环境互作研究。结果检测到27个加性QTL,16个存在显著的加性×环境互作效应位点。QTL定位分析检测到27个加性QTL,位于1、2、3、4、5、6、7、8、10号染色体上,对表型变异贡献率为1.09%~31.63%,16个QTL对表型变异贡献率大于10%,尤其是qPW一2~5单个QTL贡献率达31.63%。环境互作分析,共检测到灌浆期5个阶段的16个GE互作位点,分布在水稻2、3、4、5、6、7、8、10号染色体上,大部分为小于5%的微效QTL。此研究揭示了单穗重的动态遗传机制,为水稻穗型育种提供了依据。
Mapping quantitative trait loci(QTL) and genexenvironment interaction analysis were used to research panicle weight from a RIL including 130 lines deriving from Milyang 46 and FJCD during the grain-ffiling. This research work was carried in Wuyishan city and Putian city, Fujian province. 27 additive QTL were detected and located in chromosome 1, 2, 3, 4, 5, 6, 7, 8, 10. The contribution rates for phenotype variance were from 1.09% to 31.63%. 16 of additive QTLs could explain more than 10% of phenotypic variance, respectively, qPW-2-5 explained 31.63% of phenotypic variance. We got 16 QTLs with significant additivexenvironment interaction effects, locating in chromosome 2, 3, 4, 5, 6, 7, 8, 10; most of them were minor QTL. This study aimed to reveal dynamic expression of QTL controlling panicle weight in rice under different environmental conditions, and benefited to rice breeding for panicle type.