为考查急性低氧对鲫鱼 ( Carassius auratus L.) 幼鱼血液基础指标的影响, 将身体健康、 体重为 (267.26±13.93)g实验鱼12 尾随机平均分为两组, 对照组置于水温20、 溶氧浓度为 (7.5±0.5)mg·-1的水环境中, 处理组置于相同水温但溶氧浓度为 (1.0±0.2)mg ·-1的低氧水环境中, 两组均进行2 h处理; 测定实验鱼静脉血 pH 值、 红细胞数和血红蛋白浓度, 并计算平均红细胞血红蛋白量 (MCH ) 。结果显示, 处理组的pH 值为 (5.71±0.11) , 低于对照组的 (6.21±0.07); 处理组红细胞数为 (1.05±0.6)×10^12个·L-1, 血红蛋白浓度为 (112.0±6.7)g·L-1, 均显著高于对照组的相应指标即(0.52±0.04)×10^12和(70.9±8.6)g·L^-1;3个血液指标在处理组和对照组之间均有显著差异 (p〈0.05) ; 对照组与处理组MCH值分别为 (13.8±1.80) 、 (10.8±0.63)pg, 两者无显著差异。研究提示, 急性低氧处理可引起实验鱼血液中红细胞数、 血红蛋白浓度增加, pH 值降低, 这可能将导致血液载氧和卸氧能力的增强
This study concerns the effect of environment acute hypoxia on blood basic parameters of crucian carp ( Carassius auratus L. ) juvenile. Twelve healthy experimental fish with the body weight of (267.26 ± 13.93) g were divided randomly into 2 groups, i. e. fish in the control group were acclimated in water with a 20 ℃ temperature and 7.5 ±0.5mg · L^-1dissolved oxygen level while fish in the acute hypoxia treatment group were acclimated in water with the same temperature but 1.0 ±0.2mg·L^-1 dissolved oxygen level for 2h. The venous blood pH, red blood cell counts, hemoglobin concentrationswere measured and mean eell hemoglobin was estimated after 2h experimental treatments at 20℃. The results showed that the pH of the hypoxia-treated group( 5.71 ±0. 11 ) was lower than that of the control group (6.21 ±0.07), whereas the values of both red blood cell counts ( 1.05±0.6) × 1012 individual· L^-1 and hemoglobin concentrations ( 112.0 ±6.7) g · L^-1 in the hypoxia-treated group were higher than those of the control group, i.e. (0.52 ±0.04)×10^12 individual · L^-1for red blood cell counts and (70.9±8.6) g ·L^-1 for hemoglobin concentrations. The three blood parameters between the two experimental groups were all significantly different(p 〈 0.05). There was no significant difference in MCH between the two groups. Acute hypoxia treatment induced higher blood red blood eell counts and hemoglobin concentrations but lower blood pH in crucian carp juvenile which might enhance the capacity of blood oxygen loading and unloading capacities