中文摘要：

目的快速识别及鉴定灯心草及野灯心草中的菲类成分。方法运用高效液相色谱-电喷雾质谱（HPLC-ESI-MS）联用技术研究菲类成分的裂解规律,并分别对灯心草和野灯心草甲醇提取液中的菲类成分进行快速识别和鉴定。结果在负离子模式下,菲类成分容易丢失2H的碎片,推测可能是乙烯基与苯环环合而失去2个氢;也易发生重排丢失羰基,呈现出一定的裂解规律。根据菲类成分的裂解规律,从灯心草和野灯心草甲醇提取物中快速筛选了21个菲类成分,其中6个成分经与对照品比对而准确鉴定;另外结合质谱裂解信息及极性差异,推测出其中5个成分的结构。结论 HPLC-ESI-MS技术既可以从中药复杂体系中快速识别菲类成分并根据裂解规律初步确定菲类成分结构,也是实现中药中目标天然产物定向分离的有效手段。

英文摘要：

Objective To rapidly recognize and identify phenanthrenes in Juncus effusus and J.setchuensis.Methods HPLC-ESIMS method was used to investigate the ESI-MS characteristics of those phenanthrenes,to screen,and to identify phenanthrenes in J.effusus and J.setchuensis.Results Under negative mode,phenanthrenes were inclined to loss two hydrogens,which presumably caused by the cyclization of vinyl and aromatic ring;Furthermore,the loss of C=O could be also observed.Fragmentation pathway of phenanthrenes in ESI as a negative ion mode was summarized.Twenty-one phenanthrenes were rapidly detected from the extracts of J.effusus and J.setchuensis.Among them,six compounds were accurately identified by comparison with reference substances;The structures of five compounds were inferred according to their MS spectrum and polarity.Conclusion HPLC-ESI-MS provides not only a new technique for the rapid identification of phenanthrenes from complex matrix,but also an effective method for the target separation of constituents in Chinese materia medica.