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Establishment of AIDS Animal Model with SIVmac239 Infected Chinese Rhesus Monkey
  • ISSN号:1674-0769
  • 期刊名称:《中国病毒学:英文版》
  • 分类:R512.91[医药卫生—临床医学;医药卫生—内科学] R-332
  • 作者机构:[1]Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China, [2]Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
  • 相关基金:Key Scientific and Technological projects of China (2004BA719AI4) and Yunnan province (2004NG12, 2006PT08); National 973 project of China (2006CB504200, 2006CB504300); The Natural Science Foundation of China (30471605; 30671960); The Knowledge Innovation Program (KSCXI-YW-R-15) and "Western Light" Projects of Chinese Academy of Sciences. Acknowledgment We thank Prof. Bin Gao (Institute of Microbiology, CAS) for providing SIVmac239 molecular clone plasmids and Prof. Yun-Zhang Hu (Institute of Medical Biology, PUMC/CAMC) for providing CEMx174 cell line.
中文摘要:

在现在的研究,二中国恒河猴与 SIVmac239 的 5000 TCID50 静脉内地被接种。在 CD4 的数字的变化[+] 在外部血的 T,血浆病毒的负担,专业版病毒的脱氧核糖核酸;对病毒的体液的抗体周期性地在 121 天期间被监视。在感染的早阶段,专业版病毒的脱氧核糖核酸在 PBMC 被检测了,;传染 SIVmac239 病毒从 PBMC 被孤立。在一样的时期, CD4 的数字[+] T 显著地被减少,;在 121 天的感染期期间在底层维持了。血浆病毒的负担在星期 2 点到达了山峰接种以后;随后在一个稳恒状态保留了。而且,对病毒蛋白的抗体从血浆被检测。所有结果证明二中国恒河猴成功地感染了 SIVmac239。这个动物模型能进一步被申请爱滋病研究。

英文摘要:

In the present research, two Chinese rhesus monkeys were inoculated intravenously with 5000 TCID50 of SIVmac239. The changes in the numbers of CD4^+T lymphocyte in peripheral blood, plasma viral loads, proviral DNA and humoral antibodies against virus were periodically monitored during 121 days. At the early stage of infection, proviral DNA had been detected in PBMCs, and infectious SIVmac239 virus had been isolated from PBMCs. At the same period, the numbers of CD4^+ T lymphocytes were significantly decreased, and maintained at low level during the 121-day period of infection. Plasma viral loads reached the peak at week 2 post-inoculation and kept at a steady state subsequently. Moreover, antibodies against viral proteins were detected from plasma. All the results showed that the two Chinese rhesus monkeys had been infected with SIVmac239 successfully. This animal model can be applied for further AIDS researches.

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期刊信息
  • 《中国病毒学:英文版》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院武汉病毒研究所 中国微生物学会
  • 主编:陈新文
  • 地址:湖北省武汉市武昌区小洪山中区44号
  • 邮编:430071
  • 邮箱:info@virosin.org
  • 电话:027-87199157
  • 国际标准刊号:ISSN:1674-0769
  • 国内统一刊号:ISSN:42-1760/Q
  • 邮发代号:38-351
  • 获奖情况:
  • 湖北省第1、2、3、4、5、6届优秀期刊,中国科学技术学会 优秀国际科技期刊,2012年中国国际影响力优秀学术期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,荷兰医学文摘,美国生物医学检索系统,美国剑桥科学文摘,美国生物科学数据库,中国中国科技核心期刊
  • 被引量:208